NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE17343 Query DataSets for GSE17343
Status Public on Jul 28, 2009
Title Expression data from Arabidopsis pollen and semi in vivo- and in vitro-grown pollen tubes.
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Pollen tubes extend through pistil tissues and are guided to ovules where they release sperm for fertilization. Although pollen tubes can germinate and elongate in a synthetic medium, their trajectory is random and their growth rates are slower compared to growth in pistil tissues. Furthermore, interaction with the pistil renders pollen tubes competent to respond to guidance cues secreted by specialized cells within the ovule. The molecular basis for this potentiation of the pollen tube by the pistil remains uncharacterized.
We used a surgical procedure to obtain large quantities of uncontaminated pollen tubes that grew through the pistil and defined their transcriptome by microarray analysis. We also characterized the transcriptome of in vitro-grown pollen tubes (for 0.5hours or 4hours) and dessicated mature pollen in Arabidopsis.
 
Overall design Pollen and pollen tubes were collected as described in the protocols section for RNA extraction and hybridization on Affymetrix ATH1 Genechip microarrays.
 
Contributor(s) Qin Y, Johnson MA, Palanivelu R
Citation(s) 19714218
Submission date Jul 27, 2009
Last update date Aug 28, 2018
Contact name RAVISHANKAR PALANIVELU
E-mail(s) rpalaniv@ag.arizona.edu
Phone 520-626-2229
Fax 520-621-7186
URL http://ag.arizona.edu/research/ravilab/
Organization name University of Arizona
Department Plant Sciences
Lab Palanivelu
Street address 1140 E. South Campus Drive P.O. Box 210036
City Tucson
State/province AZ
ZIP/Postal code 85721
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (15)
GSM433634 drypollen, biological rep1
GSM433635 drypollen, biological rep2
GSM433636 drypollen, biological rep3
Relations
Affiliated with GSE69995
BioProject PRJNA118993

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17343_RAW.tar 28.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap