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Series GSE173658 Query DataSets for GSE173658
Status Public on May 01, 2021
Title Genome-wide expression changes in response to the treatment of anti-CD93 in mouse tumor endothelial cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We isolated ECs from anti-CD93 antibody treated or control KPC tumor and performed RNA-seq to investigate genome-wide expression changes in response to the treatment of anti-CD93. Many of the genes affected by anti-CD93 were functionally related to cell adhesion, angiogenesis or vasculature development. Gene ontology analysis further confirmed that the transcriptomic changes by anti-CD93 were mainly related to biological pathways of angiogenesis, cell adhesion, or vasculature development.
Overall design Total RNA was extracted and purified using QIAGEN RNeasy Mini Kit (Cat.74104) from tumor endothelial cells, which were isolated by FACS sorting (CD31+CD45- population) from established subcutaneous KPC tumor after 48 hours of antibody treatment. The mRNA libraries were constructed using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Cat. 634411). 150bp paired-end RNA sequencing was performed on Illumina NovaSEQ 6000 platform and the RNA-seq data were generated by the Genomics and Microarray Shared Resource at UCCC.
Contributor(s) Zhu Y, Sun Y
Citation(s) 34321321
Submission date Apr 30, 2021
Last update date Jan 20, 2022
Contact name Yi Sun
Phone 7204218675
Organization name UC Denver
Department Surgery
Lab Surgical Oncology
Street address 12800 E 19th Ave | RC1N 8F | P18-8402D
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM5273203 tumor endothelial cells - Rat IgG treated -1
GSM5273204 tumor endothelial cells - Rat IgG treated -2
GSM5273205 tumor endothelial cells - anti-CD93 treated -1
BioProject PRJNA726551
SRA SRP318027

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Supplementary file Size Download File type/resource
GSE173658_final_counts.txt.gz 3.9 Mb (ftp)(http) TXT
GSE173658_normalized_counts.txt.gz 382.5 Kb (ftp)(http) TXT
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