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| Status |
Public on May 01, 2021 |
| Title |
Genome-wide expression changes in response to the treatment of anti-CD93 in mouse tumor endothelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We isolated ECs from anti-CD93 antibody treated or control KPC tumor and performed RNA-seq to investigate genome-wide expression changes in response to the treatment of anti-CD93. Many of the genes affected by anti-CD93 were functionally related to cell adhesion, angiogenesis or vasculature development. Gene ontology analysis further confirmed that the transcriptomic changes by anti-CD93 were mainly related to biological pathways of angiogenesis, cell adhesion, or vasculature development.
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| Overall design |
Total RNA was extracted and purified using QIAGEN RNeasy Mini Kit (Cat.74104) from tumor endothelial cells, which were isolated by FACS sorting (CD31+CD45- population) from established subcutaneous KPC tumor after 48 hours of antibody treatment. The mRNA libraries were constructed using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Cat. 634411). 150bp paired-end RNA sequencing was performed on Illumina NovaSEQ 6000 platform and the RNA-seq data were generated by the Genomics and Microarray Shared Resource at UCCC.
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| Contributor(s) |
Zhu Y, Sun Y |
| Citation(s) |
34321321 |
| Submission date |
Apr 30, 2021 |
| Last update date |
Jan 20, 2022 |
| Contact name |
Yi Sun |
| E-mail(s) |
yi.2.sun@cuanschutz.edu
|
| Phone |
7204218675
|
| Organization name |
UC Denver
|
| Department |
Surgery
|
| Lab |
Surgical Oncology
|
| Street address |
12800 E 19th Ave | RC1N 8F | P18-8402D
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| GSM5273203 |
tumor endothelial cells - Rat IgG treated -1 |
| GSM5273204 |
tumor endothelial cells - Rat IgG treated -2 |
| GSM5273205 |
tumor endothelial cells - anti-CD93 treated -1 |
| GSM5273206 |
tumor endothelial cells - anti-CD93 treated -2 |
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| Relations |
| BioProject |
PRJNA726551 |
| SRA |
SRP318027 |
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