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| Status |
Public on May 03, 2021 |
| Title |
MicroRNA-seq of MDA-MB-231 cells transduced with anti-ELOVL5 and control shRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
MiRNA isoforms (isomiRs) are single stranded small RNAs originating from the same pri-miRNA hairpin as a result of cleavage by Drosha and Dicer enzymes. Variations at the 5’-end of a miRNA alter the seed region of the molecule, thus affecting the targetome of the miRNA. MDA-MB-231 cells were transduced with shRNAs against ELOVL5 and luciferase genes. Small RNA sequencing was used to study the cleavage profiles of shRNAs.
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| Overall design |
Sequencing was performed for two samples: MDA-MB-231 cells trancduced with shELOVL5 and MDA-MB-231 cells trancduced with shLuc.
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| Contributor(s) |
Zhiyanov A, Nersisyan S, Tonevitsky A |
| Citation(s) |
34286662 |
| Submission date |
May 02, 2021 |
| Last update date |
Aug 18, 2021 |
| Contact name |
Stepan Nersisyan |
| E-mail(s) |
s.a.nersisyan@gmail.com
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| Organization name |
HSE University
|
| Department |
Faculty of Biology and Biotechnology
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| Street address |
Vavilova str 7
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| City |
Moscow |
| ZIP/Postal code |
117312 |
| Country |
Russia |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA726811 |
| SRA |
SRP318154 |
