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| Status |
Public on May 24, 2022 |
| Title |
ATAC-seq of immature B cells in presence or absence of γδ T cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Purpose: compare immature B cells in presence or not of γδ T cells in vivo and in vitro.
Methods: immature B cells were sorted from spleen of control Tcrd-GDL mice and Tcrd-GDL mice treated with Diphteria toxin (DTx) for depletrion of γδ T cells after eight days and were sequenced for transcriptional (RNA-seq) and epigenetic profile (ATAC-seq). Further, immature B cells were sorted from spleen of Tcdr-H2B mice, cultured alone or in presence of γδ T cells and sequenced for transcriptional profile (RNA-seq).
Results/Conclusions: In presence of γδ T cells, immature B cells upregulated genes related to unfolded protein response and mTORC1 signaling
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| Overall design |
Transcriptional and epigenetic profile of splenic immaure B cells isolated from control mice and mice depleted for eight days of γδ T cells; transcriptional profile of immature B cells cultured alone or in presence of γδ T cells
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| Contributor(s) |
Rampoldi F, Donato E |
| Citation(s) |
35613579 |
| Submission date |
May 03, 2021 |
| Last update date |
Aug 25, 2022 |
| Contact name |
Elisa Donato |
| E-mail(s) |
e.donato@dkfz.de
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| Organization name |
Deutsches Krebsforschungszentrum (DKFZ) - German Cancer Research Center
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| Department |
Division of Stem Cells and Cancer
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| Lab |
Prof. Dr. Andreas Trumpp
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| Street address |
Im Neuenheimer Feld 280
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE174564 |
RNA-seq and ATAC-seq of immature B cells in presence or absence of γδ T cells |
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| Relations |
| BioProject |
PRJNA726952 |
| SRA |
SRP318219 |
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