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| Status |
Public on Jan 20, 2022 |
| Title |
Loss of Slc38a4 imprinting is a major cause of mouse SCNT placenta hyperplasia at late gestation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Placenta hyperplasia is commonly observed in cloned animals and is believed to impede the proper development of cloned embryos. However, the mechanism underlying this phenomenon is largely unknown. Here we show that placenta hyperplasia of cloned mouse embryos occurs in both middle and late gestation. Interestingly, restoring paternal-specific expression of an amino acid transporter Slc38a4, which loses maternal H3K27me3-dependent imprinting and becomes bi-allelically expressed in cloned placentae, rescued the overgrowth of cloned placentae at late gestation. Molecular analyses reveal that loss of Slc38a4 imprinting leads to over activation of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway in cloned placentae, which is likely due to the increased amino acids transportation by SLC38A4. Collectively, our study not only reveals loss of Slc38a4 imprinting is responsible for overgrowth of cloned placentae at late gestation, but also suggests the underlying mechanism involves increased amino acid transportation and over activation of mTORC1.
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| Overall design |
We conducted RNA sequencing in in vitro fertilized (IVF), wild type SCNT and Slc38a4 maternal knockout SCNT placenta at E14.5 and E19.5
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| Contributor(s) |
Xie Z, Zhang W, Zhang Y |
| Citation(s) |
35196486 |
| Submission date |
May 10, 2021 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Wenhao Zhang |
| E-mail(s) |
zwhonep2@gmail.com
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| Phone |
+1 617-784-1683
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| Organization name |
Harvard Medical School
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| Department |
Genetics
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| Lab |
Yi Zhang
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| Street address |
Warren Alpert Bldg, Rm 160, 200 Longwood Ave
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA728675 |
| SRA |
SRP319189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE174174_RAW.tar |
2.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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