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Series GSE174287 Query DataSets for GSE174287
Status Public on May 12, 2021
Title Gene Expression Profile From 15 day Embryonic Mouse Eyes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Absence of Ocular albinism type 1 (Oa1) gene is associated with abnormal pigmentation and misrouting of retinal ganglion cell (RGC) axons at the optic chiasm of the brain. Expression of the Oa1 gene is tightly correlated with development of RGCs. In mice the Oa1 gene is expressed at embryonic day (E) 10.5. Soon afterwards the RGCs appear and at E15.5 majority of their axons pass through the optic chiasm. So comparison of microarrays hybridized with mRNAs from E15.5 Ocular albino (Oa1-/-) and Wild Type (WT) mice eyes will help us identify candidate genes critical for Ocular albinism.
We used microarray analysis to study the differential expression of genes between E15.5 eyes from Wild type and Oa1-/- mice.
Overall design Six pregnant females, three Oa1-/- and three WT, were sacrificed at day 15.5 days post coitum. Eyes from six embryos from each pregnant mouse were isolated and treated as one biological sample. After enucleation, the anterior segments were dissected out. RNA was extracted from the posterior eye segments of each of 3 Oa1-/- (T1, T2, T3) and 3 WT (C1, C2, C3) sample and hybridized to Affymetrix microarrays.
Contributor(s) Guha S, Farber D
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Submission date May 11, 2021
Last update date May 14, 2021
Contact name SONIA GUHA
Organization name UCLA
Department Ophthalmology, SEI
Street address 650 Charles E Young Dr, S
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM5291043 E15.5 WT eyes, biological replicate 1
GSM5291044 E15.5 WT eyes, biological replicate 2
GSM5291045 E15.5 WT eyes, biological replicate 3
BioProject PRJNA729104

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Supplementary file Size Download File type/resource
GSE174287_RAW.tar 24.9 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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