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| Status |
Public on Sep 01, 2009 |
| Title |
Expression data in WT and ES cells overexpressing Satb1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Satb1 and Satb2 are regulators of higher order chromatin in T cells and osteoblasts repectively. We were interested if Satb1 and Satb2 play a role in the regulation of gene expression in ES cells.
ES cells which can inducibly express Satb1 or Satb2 were generated using the tet-ON system. In this experiment, we compared gene expression in uninduced cells to that in cells which had been induced for 24h to express Satb1 ectopcially at very high levels.
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| Overall design |
Cells were cultured, passaged every 3 days. After trypsinisation cells were grown for 1 day in the presence of LIF only, after 1 day a set of cultures was induced by the addition of DOX to the culture medium. Induction took place for 24h, RNA was generated 48h after passaging.
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| Contributor(s) |
Savarese F, Engelke R, Nechanitzky R, Grosschedl R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 03, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Rudolf Engelke |
| E-mail(s) |
engelke@immunbio.mpg.de
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| Phone |
+49 761 5108 751
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| Organization name |
Max Planck Institute of Immunobiology
|
| Street address |
Stuebeweg 51
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| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE17490 |
Role of Satb1 and Satb2 in embryonic stem cells |
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| Relations |
| BioProject |
PRJNA123629 |
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