|
| Status |
Public on Jun 04, 2021 |
| Title |
Endothelial cell infection and dysfunction, immune activation in severe COVID-19 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
SARS-CoV2 infects endothelial cells and induce the dysfunction of them.
|
| |
|
| Overall design |
I. We infected WT C57BL/6J or K18 hACE2 transegnic mice in both male and female mice with 2x10^5 pfu SARS-CoV2. Then those mice were eutanized 4 days post challenge and the lungs were removed and processed for total RNA preparation.
II. We infected female WT C57BL/6J or K18 hACE2 transgenic mice with 2x10^5 pfu SARS-CoV2. Then those mice were eutanized 4 days post challenge and the lungs were removed and processed for single cell suspensions.
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| |
|
| Contributor(s) |
Qin X, Kolls J |
| Citation(s) |
34335981 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U54 CA260581 |
Tulane University COVID Antibody and Immunity Network (TUCAIN) |
TULANE UNIVERSITY |
JAMES E Robinson |
|
| Submission date |
Jun 02, 2021 |
| Last update date |
Aug 01, 2023 |
| Contact name |
Jay Kolls |
| Organization name |
Tulane University
|
| Department |
Medicine
|
| Lab |
Center for Translational Research in Infection and Inflammation
|
| Street address |
1430 Tulane Ave
|
| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21626 |
NextSeq 550 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA734526 |
| SRA |
SRP322333 |
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