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Series GSE17624 Query DataSets for GSE17624
Status Public on Aug 13, 2009
Title Expression data from human Ishikawa cells treated with Bisphenol A
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This study provides a comprehensive evaluation of changes in gene expression during treatment with Bisphenol A in vitro.
Ishikawa cells were maintained and grown (to confluency or at a desired cell density between 500,000 - 1 million cells/mL) in DMEM/F-12 Medium (supplemented with 10% Fetal Bovine Serum + 1X penicillin/streptomycin) in xenoestrogen-free Corning plasticware. Cells were gently washed in warm PBS and transferred to phenol red-free DMEM/F-12 Medium (supplemented with 10% Charcoal-Stripped Fetal Bovine Serum + 1X penicillin/streptomycin) in Corning Cell Culture Cluster wells overnight and then challenged with 1pM (very low, vL), 100pM (low, L), 10nM (high, H), and 1uM (very high, vH) levels of BPA. Prior to collection, cells were washed in warm PBS, resuspended & briefly incubated in TRI-Reagent, & finally collected (in quintuplicate replicates) at each specific time point: 8 hours, 24 hours, and 48 hours. Following RNA isolation, the best RNA yields for each quadruplicate set was selected for target preparation and GeneChip processing.

Keywords: Dose Response/Time Course
 
Overall design 60 total samples: 5 Doses [Vehicle Control, Very Low (1 nM), Low (100 nM), High (10 uM), Very High (100 uM)]; 3 Timepoints [8 hour, 24 hour, 48 hour]; 4 Replicates each.
 
Contributor(s) Tiesman JP
Citation(s) 20170705
Submission date Aug 12, 2009
Last update date Mar 25, 2019
Contact name Jay Patrick Tiesman
Organization name Procter & Gamble
Department Global Biotechnology
Lab Mason Business Center
Street address PO BOX 8006
City MASON
State/province OH
ZIP/Postal code 45040-8006
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (60)
GSM439987 Ishikawa_Control_8h_rep1
GSM439988 Ishikawa_Control_8h_rep2
GSM439989 Ishikawa_Control_8h_rep3
Relations
BioProject PRJNA118613

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17624_RAW.tar 320.7 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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