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Series GSE17635 Query DataSets for GSE17635
Status Public on Dec 18, 2009
Title Folic acid supplementation normalizes the endothelial progenitor cell transcriptome of patients with type 1 diabetes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background: Endothelial progenitor cells play an important role in vascular wall repair. Patients with type 1 diabetes have reduced levels of endothelial progenitor cells of which their functional capacity is impaired. Reduced nitric oxide bioavailability and increased oxidative stress play a role in endothelial progenitor cell dysfunction in these patients. Folic acid, a B-vitamin with anti-oxidant properties, may be able to improve endothelial progenitor cell function. In this study, we investigated the gene expression profiles of endothelial progenitor cells from patients with type 1 diabetes compared to endothelial progenitor cells from healthy subjects. Furthermore, we studied the effect of folic acid on gene expression profiles of endothelial progenitor cells from patients with type 1 diabetes. Methods: We used microarray analysis to investigate the gene expression profiles of endothelial progenitor cells from type 1 diabetes patients before (n=11) and after a four week period of folic acid supplementation (n=10) compared to the gene expression profiles of endothelial progenitor cells from healthy subjects (n=11). The probability of genes being differentially expressed among the classes was computed using a random-variance t-test. A multivariate permutation test was used to identify genes that were differentially expressed among the two classes. Functional classification of differentially expressed genes was performed using the biological process ontology in the Gene Ontology database. Results: Type 1 diabetes significantly modulated the expression of 1591 genes compared to healthy controls. These genes were found to be involved in processes regulating development, cell communication, cell adhesion and localization. After folic acid treatment, endothelial progenitor cell gene expression profiles from diabetic patients were similar to those from healthy controls. Genes that were normalized by folic acid played a prominent role in development, such as the transcription factors ID1 and MAFF. Few oxidative-stress related genes were affected by folic acid. Conclusion: Folic acid normalizes endothelial progenitor cell gene expression profiles of patients with type 1 diabetes. Signaling pathways modulated by folic acid may be potential therapeutic targets to improve endothelial progenitor cell function.
 
Overall design We used microarray analysis to investigate the gene expression profiles of endothelial progenitor cells from type 1 diabetes patients before (n=11) and after a four week period of folic acid supplementation (n=10) compared to the gene expression profiles of endothelial progenitor cells from healthy subjects (n=11)
 
Contributor(s) van Oostrom O, de Kleijn DP, Fledderus JO, Pescatori M, Stubbs A, Tuinenburg A, Lim S, Verhaar MC
Citation(s) 19706161
Submission date Aug 13, 2009
Last update date Mar 21, 2012
Contact name Mario Pescatori
E-mail(s) mariopescatori@gmail.com
Phone 00393930761013
Organization name Health e-genomics
Street address Westplein 11
City rotterdam
ZIP/Postal code 3016BM
Country Netherlands
 
Platforms (1)
GPL2700 Sentrix HumanRef-8 Expression BeadChip
Samples (32)
GSM440157 Patient 1
GSM440158 Patient 2
GSM440159 Patient 3
Relations
BioProject PRJNA118573

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17635_non_normalized.txt.gz 1.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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