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Series GSE176471 Query DataSets for GSE176471
Status Public on Jun 10, 2021
Title Cell cycle dysregulation with overexpression of KIF2C/MCAK is a critical event in nasopharyngeal carcinoma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Nasopharyngeal carcinoma (NPC) is a common malignant carcinoma of the head and neck, and the biological mechanisms underlying the pathogenesis of NPC remain not fully understood.Thus, more studies are needed to identify novel critical molecular events and key hub genes to develop novel diagnostic and therapeutic strategies for the management and treatment of NPC.
Methods: In the present study, we systematically analyzed four independent NPC transcriptomic da-tasets and focused on identifying the critical molecular networks and novel key hub genes implicated in NPC. We found totally 170 common overlapping differentially expressed genes (DEGs) in the four NPC datasets. GO and KEGG pathway analysis revealed that cell cycle dysregulation is a critical event in NPC. Protein-protein interaction (PPI) network analysis identified a 15 hub-gene core network with overexpressed kinesin family member 2C (KIF2C) as a central regulator.
Results: Loss-of-function study demonstrated that knockdown of KIF2C significantly inhibited cell growth and cell motility, and delayed cell cycle progression, accompanied with dramatic mitotic defects in spindle formation in NPC cells. RNA-seq analysis revealed that KIF2C knockdown led to deregulation of various downstream genes. KIF2C could also regulate the AKT/mTOR pathways, and enhance paclitaxel sensitivity in NPC cells.
Conclusions: Taken together, our results suggest that cell cycle dysregulation is a critical event during NPC pathogenesis and KIF2C is a novel key mitotic hub gene with therapeutic potential in NPC.
 
Overall design mRNA profiles of NCsh and KIF2Csh in HNE-1 cells
 
Contributor(s) Zuo X, Meng P
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Submission date Jun 09, 2021
Last update date Jun 10, 2021
Contact name Chuntao Tao
Organization name Chongqing Medical University
Street address China
City Chongqing
State/province State...
ZIP/Postal code 408500
Country China
 
Platforms (1)
GPL30209 MGISEQ-2000RS (Homo sapiens)
Samples (2)
GSM5366134 HNE-1 cells, NCsh
GSM5366135 HNE-1 cells, KIF2Csh
Relations
BioProject PRJNA736377
SRA SRP323373

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Supplementary file Size Download File type/resource
GSE176471_genes_fpkm_expression.xlsx 508.4 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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