NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE17698 Query DataSets for GSE17698
Status Public on Aug 19, 2009
Title The genetics of wound healing in tympanic membranes in rats
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The aim of this study was to identify the genes differentially expressed between timepoints in the week following tympanic membrane perforation in rats. Tissue from 240 individual rats was used in this study following random allocation into timepoint groups to be sacrificed over 7 days. An Agilent one color microarray technique was performed and the results were analyzed using Genespring GX9 software. A total of 3262 genes were identified as significant (p<0.05) and differentially expressed above a two-fold threshold between the timepoints. This study provides a complete genetic review of rat tympanic membrane wound healing over 7 days. The results can be used as a model for other wound healing in other mammals and in different parts of the body. The information on differential gene expression can be used in research towards developing chronic tympanic membrane perforations and also in research to treat acute and chronic tympanic membrane perforations. The microarray was performed on animals in a disease free environment and the genetic information can be compared to future research in disease states of the TM including Otitis media, cholesteatoma, chronic perforation and tympanosclerosis.
 
Overall design Rats were randomly selected as either controls or in the perforation group. Perforations were created unilaterally (left ear) in the upper outer quadrant of the pars tensa of rats’ tympanic membranes using sterile 23 gauge needles . Rats were then randomly allocated into timepoint groups to be sacrificed at either 12, 24, 36, day 2, 3, 4, 5, 6, 7. At the point of microarray, there were 18 rats per timepoint group and 18 controls.
 
Contributor(s) SantaMaria PL, Redmond SL, McInnes RL, Atlas MD, Ghassemifar R
Citation(s) 21919009
Submission date Aug 18, 2009
Last update date Dec 21, 2016
Contact name Peter Luke Santa Maria
Organization name University of Western Australia
Department Ear Sciences
Street address 2nd floor M Block, QE II Medical Centre, Nedlands
City Perth
State/province WA
ZIP/Postal code 6009
Country Australia
 
Platforms (1)
GPL4135 Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Feature Number version)
Samples (40)
GSM441288 tympanic membrane_perforated_36hr_group2
GSM441289 tympanic membrane_perforated_48hr_group3
GSM441290 tympanic membrane_perforated_144hr_group2
Relations
BioProject PRJNA118403

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17698_RAW.tar 347.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap