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Series GSE17730 Query DataSets for GSE17730
Status Public on Jan 01, 2010
Title Hepatic transcriptome of KK/H1J diabetic mice treated with bisphosphatase deficient PFK-2 adenovirus
Organism Mus musculus
Experiment type Expression profiling by array
Summary In an effort to reduce hepatic glucose production and lower plasma glucose levels that are characteristics of diabetes, we have devised a treatment whereby we enhance glycolysis in the liver of a type 2 diabetic mouse model (KK/H1J). We achieve this by raising the levels of a potent regulator of glucose metabolism, fructose-2,6-bisphosphate (F26BP). Treating the mice in this way, we have demonstrated amelioration of the diabetic phenotype in terms of lowering plasma glucose and insulin levels. These treated mice also display reduced weight gain, reduced adiposity, and normalized plasma and hepatic lipid levels. These latter metabolic changes brought about by raising F26BP levels are counterintuitive. A concurrent increase in glycolysis and lipogenesis is expected, however, we observe an increase in glycolysis with a concurrent decrease in lipogenesis. Preliminary analysis of gene expression in these mice has revealed alterations in gene expression of several genes that support the therapeutic effect of raising F26BP levels. To further profile F26BP effects on hepatic gene expression, we have applied a comprehensive approach to identify sets of genes and thereby biological pathways that are differentially regulated when hepatic glycolysis is accelerated in diabetic mice. Comparing diabetic and treated animals, we hope to further clarify the molecular and genetic signature of type 2 diabetes and obesity and elucidate how this signature is affected by raising F26BP levels. Our study examining gene array as well as the hepatic proteome has identified multiple gene and protein expression changes. Of particular relevance, we note that fatty acid metabolism and cholesterol metabolism pathways are significantly down-regulated in diabetic mice treated with a PFK-2 mutant engineered to raise F26BP levels, which underlie the changes we see in the metabolic parameters.
 
Overall design 6 KK/H1J mice, a polygenic model of type 2 diabetes and obesity, weighing approximately 35g were used in this study. 3 mice were injected with GFP control adenovirus and 3 mice were injected with bisphosphatase deficient PFK-2 adenovirus (BPD). Mice were followed for 7 days before livers were extracted. Total cellular RNA was extracted from each mouse and analyzed by microarray separately.
 
Contributor(s) Khan SA, Wu C, Mulukutla B, Hu W, Lange AJ
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Submission date Aug 19, 2009
Last update date May 04, 2018
Contact name Salmaan A Khan
E-mail(s) khan0173@umn.edu
Organization name University of Minnesota
Department BMBB
Lab Lange
Street address 420 Washington Ave, 7-162 MCB Building
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (6)
GSM442607 BPD treated KK/H1J mouse liver number 23
GSM442608 BPD treated KK/H1J mouse liver number 28
GSM442609 BPD treated KK/H1J mouse liver number 36
Relations
BioProject PRJNA118379

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Supplementary file Size Download File type/resource
GSE17730_RAW.tar 13.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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