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Series GSE17733 Query DataSets for GSE17733
Status Public on May 31, 2011
Title Delineation of EZH2 oncogenic functions in hepatocellular carcinoma
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary The goal of this study was to delineate the important EZH2 direct target genes that mediate the oncogenic properties of EZH2 in HCC. The EZH2 direct target genes in two HCC cell lines were identified by chromatin immunoprecipitation microarray (ChIP-chip) analysis and later confirmed by independent ChIP-PCR. The functions of the target genes were further examined.
 
Overall design Two human HCC cell lines, Huh7 and PLC/PRF/5 (PLC5), were grown in DMEM supplemented with 10% fetal bovine serum. ChIP assays were performed using anti-EZH2 antibody. The immunoprecipitated and input DNA were used to probe the Agilent human ChIP-chip arrays.
 
Contributor(s) Cheng AS, Choy RK
Citation(s) 21512140
Submission date Aug 20, 2009
Last update date Dec 06, 2012
Contact name Richard KW CHOY
E-mail(s) richardchoy@cuhk.edu.hk
Phone 852-26323099
Fax 852-26360008
Organization name The Chinese University of Hong Kong
Department Obstetrics & Gynaecology
Street address 1/F, Block E, Prince of Wales Hospital
City Shatin
ZIP/Postal code SAR
Country Hong Kong
 
Platforms (2)
GPL4124 Agilent-014706 Human Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 G4489A (Feature Number version)
GPL4125 Agilent-014707 Human Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 G4489A (Feature Number version)
Samples (8)
GSM442662 Huh7 EZH2 ChIP-chip Replicate 1 Slide 1
GSM442663 Huh7 EZH2 ChIP-chip Replicate 1 Slide 2
GSM442664 Huh7 EZH2 ChIP-chip Replicate 2 Slide 1
Relations
BioProject PRJNA118385

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17733_RAW.tar 549.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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