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| Status |
Public on Jun 16, 2021 |
| Title |
Rprd Proteins Control Transcription In Human Cells |
| Organisms |
Saccharomyces cerevisiae; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Regulation of transcription is an essential process that allows the cell to respond to various internal and external signals. RNA Polymerase II (Pol II) activity is controlled by a number of factors which bind to the C-terminal domain (CTD) of its largest subunit, RPB1, and stimulate or supress RNA synthesis. Here, we demonstrate that members of the RPRD family of CTD-interacting proteins, RPRD1A, RPRD1B and RPRD2, act as negative regulators of transcription. We show they form mutually exclusive complexes with Pol II, coordinating this way their roles in transcription control. Our data indicate that the main alterations in gene expression patterns are driven by RPRD2, which results in further consequences during the cellular stress response, cell cycle and cell growth.
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| Overall design |
Investigation the role of RPRD proteins on newly synthesized RNA
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| Contributor(s) |
Winczura K, Ceylan H, Sledziowska M, Jones M, Fagarasan H, Wang J, Saporano M, Hebenstreit D, Arnold R, Grzechnik P |
| Citation missing |
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| Submission date |
Jun 15, 2021 |
| Last update date |
Jun 19, 2021 |
| Contact name |
Hurmuz CEYLAN |
| E-mail(s) |
HXC896@student.bham.ac.uk
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| Organization name |
University of Birmingham
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| Street address |
School of Biosciences, University of Birmingham,, Edgbaston B15 2TT
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| City |
BIRMINGHAM |
| ZIP/Postal code |
B152TT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20276 |
Illumina NextSeq 500 (Homo sapiens; Saccharomyces cerevisiae) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA737762 |
| SRA |
SRP324104 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178213_RAW.tar |
657.9 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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