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| Status |
Public on Apr 21, 2010 |
| Title |
Metabolically engineered urea degrading and urea importing Sake yeast strains K7 (WT), K7 Dur1,2 and K7 Dur3 |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Excess/residual urea is a pervasion problem in wine and Sake fermentation. We sought to reduce residual urea levels (to reduce ethyl carbamate leves) by engineering the Sake yeast strain K7 to constitutively express either the urea amidolyase (Dur1,2) or urea importer (Dur3). We sought to then compare the gene expression profiles of the metabolically engineered yeast strains to the parental strain during fermentation.
Engineered strains would hopefully have gene expression profiles that were minimally different from the parental strain.
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| Overall design |
Yeast strains were used to ferment Chardonnay grape must and total RNA harvested at 24 hrs into fermentation. 10 ug of total RNA was made into cDNA, and then labelled cRNA, with the Affymetrix GeneChip one cycle target amplification and labelling system. Fragmented cRNA was then hybridized to an Affymetrix YGS98 array in biological duplicate.
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| Contributor(s) |
Dahabieh MS, Luo Z |
| Citation(s) |
20408912 |
| Submission date |
Aug 28, 2009 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Matthew Dahabieh |
| E-mail(s) |
dahabieh@interchange.ubc.ca
|
| Organization name |
University of British Columbia
|
| Department |
Wine Research Centre
|
| Lab |
van Vuuren
|
| Street address |
2205 East Mall
|
| City |
Vancouver |
| ZIP/Postal code |
V6T1Z4 |
| Country |
Canada |
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| Platforms (1) |
| GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA120115 |
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