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| Status |
Public on Feb 24, 2010 |
| Title |
Sex-related transcriptional differences in Day 7 bovine in vitro produced blastocysts |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
|
| Summary |
Sexual dimorphism in mammals is mostly attributable to sex-related hormonal differences in fetal and adult tissues; however, this may not be the sole determinant. Though genetically-identical for autosomal chromosomes, male and female preimplantation embryos could display sex-specific transcriptional regulation which can only be attributted to the differences in sexual chromosome dosage.
We used microarrays to analyze sex-related transcriptional differences at the blastocyst stage.
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| Overall design |
Day 7 bovine in vitro produced bovine blastocysts produced with sorted semen from 3 different bulls. Pooled RNA from 60 blastocysts of one sex and produced with one bull was used per chip. Three replicates of each sex per bull. In total, 18 Bovine GeneChip (Affymetrix) were used (3 replicates X 3 bulls X 2 sexes).
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| Contributor(s) |
Bermejo-Álvarez P, Rizos D, Rath D, Lonergan P, Gutiérrez-Adán A |
| Citation(s) |
20133684 |
| Submission date |
Sep 01, 2009 |
| Last update date |
Dec 17, 2012 |
| Contact name |
Pablo Bermejo-Álvarez |
| E-mail(s) |
borrillobermejo@hotmail.com
|
| Phone |
0034-913473768
|
| Fax |
0034-913474014
|
| Organization name |
INIA
|
| Department |
Reproducción Animal
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| Lab |
Molecular Embryology
|
| Street address |
Ctra La Coruña Km 5,9
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28039 |
| Country |
Spain |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA119881 |
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