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| Status |
Public on Jul 02, 2021 |
| Title |
Changes in splicing and neuromodulatory gene expression programs in sensory neurons with pheromone signaling and social experience |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Social experience and pheromone signaling in ORNs affect pheromone responses and male courtship behaviors in Drosophila, however, the molecular mechanisms underlying this circuit-level neuromodulation remain less clear. Previous studies identified social experience and pheromone signaling-dependent modulation of chromatin around behavioral switch gene fruitless, which encodes a transcription factor necessary and sufficient for male behaviors. To identify the molecular mechanisms driving social experience-dependent neuromodulation, we performed RNA-seq from antennal samples of mutant fruit flies in pheromone receptors and fruitless, as well as grouped or isolated wild-type males. We found that loss of pheromone detection differentially alters the levels of fruitless exons suggesting changes in splicing patterns. In addition, many Fruitless target neuromodulatory genes, such as neurotransmitter receptors, ion channels, and ion transporters, are differentially regulated by social context and pheromone signaling. Our results suggest that modulation of circuit activity and behaviors in response to social experience and pheromone signaling arise due to changes in transcriptional programs for neuromodulators downstream of behavioral switch gene function.
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| Overall design |
RNA-seq using whole third segment antennae from Drosophila melanogaster. One biological replicate includes 180 antennae from 90 flies at 7 days old.
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| Contributor(s) |
Deanhardt B, Duan Q, Du C, Soeder C, Jones C, Volkan PC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 30, 2021 |
| Last update date |
Jul 13, 2021 |
| Contact name |
Corbin Jones |
| E-mail(s) |
cdjones@email.unc.edu
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| Organization name |
UNC-CH
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| Department |
Biology
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| Street address |
250 Bell Tower Dr
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| City |
Chapel Hil |
| State/province |
NC |
| ZIP/Postal code |
27514 |
| Country |
USA |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (15)
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| GSM5411547 |
Seven days old, 47b1 mutant - replicate 1 |
| GSM5411548 |
Seven days old, 47b1 mutant - replicate 2 |
| GSM5411549 |
Seven days old, 47b1 mutant - replicate 3 |
| GSM5411550 |
Seven days old, 67d mutant - replicate 1 |
| GSM5411551 |
Seven days old, 67d mutant - replicate 2 |
| GSM5411552 |
Seven days old, 67d mutant - replicate 3 |
| GSM5411553 |
Seven days old, white-eyed group housed - replicate 1 |
| GSM5411554 |
Seven days old, white-eyed group housed - replicate 2 |
| GSM5411555 |
Seven days old, white-eyed group housed - replicate 3 |
| GSM5411556 |
Seven days old, white-eyed social isolation - replicate 1 |
| GSM5411557 |
Seven days old, white-eyed social isolation - replicate 2 |
| GSM5411558 |
Seven days old, white-eyed social isolation - replicate 3 |
| GSM5411559 |
Seven days old, FruLexA/Fru440 mutant - replicate 2 |
| GSM5411560 |
Seven days old, FruLexA/Fru440 mutant - replicate 3 |
| GSM5435111 |
Seven days old, FruLexA/Fru440 mutant - replicate 1 |
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| Relations |
| BioProject |
PRJNA742720 |
| SRA |
SRP326322 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE179213_FRULEXAFRU440_1_new_sequence_update.vs_dm6main.dm6_genes.mapspliceMulti.MpBC.counts.txt.gz |
837.9 Kb |
(ftp)(http) |
TXT |
| GSE179213_new_sequence.vs_dm6main.dm6_genes.mapspliceMulti.MpBC.counts.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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