C57BL/6 wild type mice were exposed to 12 Gy whole-body irradiation. Before irradiation (t=0) and at t=24, 48 and 96 hours after irradiation, mice (n=10 per time point) were euthanized. From n=5 mice per time point RNA was isolated from small-intestinal (jejunum) tissue, from n=5 mice per time point RNA was isolated from EDTA-isolated epithelial cells from small-intestinal tissue (jejunum).
Overall design
n=40 in total: n=5 small intestinal tissue per time point, n=5 EDTA-isolated small intestinal epithelial cells per time point. Time points t=0h, 24h, 48h, 96h