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| Status |
Public on Jul 20, 2021 |
| Title |
mRNA-sequencing of METTL3-deficient NK cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: For comparing the transcript changes, we conducted mRNA-sequencing of splenic NK cells from Ncr1Cre-Mettl3fl/fl (cKO) and Mettl3fl/fl (WT) mice.
Method: Firstly, The splenic NK cells (CD45.2+CD3-NK1.1+NKp46+) are purified via Fluorescence activated Cell Sorting (FACS), then frozen in -80 °C ultra-low temperature refrigerator, followed by High-throughput sequencing, in three replicates, using Illumina Hiseq 1500 platform.
Result: Using standard data process workflow, we found the differentailly expressed genes in NK cells from Ncr1Cre-Mettl3fl/fl (cKO) mice, compared with those from Mettl3fl/fl (WT) mice.
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| Overall design |
Find the DEGs in cKO NK cells.
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| Contributor(s) |
Song H |
| Citation(s) |
34535671 |
| Submission date |
Jul 13, 2021 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Zhigang Tian |
| E-mail(s) |
tzg@ustc.edu.cn
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| Phone |
+8615665425083
|
| Organization name |
Univerity of Science and Technology of China
|
| Street address |
Huangshan Road，number 443
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230000 |
| Country |
China |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA746336 |
| SRA |
SRP328215 |
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