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| Status |
Public on Oct 21, 2021 |
| Title |
Single cell RNA-sequencing of long-term human control and C9ORF72 ALS/FTD patient-specific iPSC-derived cortical organoid slice cultures |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell RNA sequencing data was collected from 150 day old human cortical organoid slice cultures grown at the air-liquid interface (ALI-COs) from control and ALS/FTD patient-specific induced pluripotent stem cell (iPSC) lines harbouring the C9ORF72 hexanucleotide repeat expansion mutation. Data collection included ALI-COs derived from the following iPSC lines: WTSli042-B (WTS42b) and A18945 (EpiC) for healthy controls, CS30iALS-C9nxx (CS30) and CS29iALS-C9nxx (CS29) for ALS/FTD, and CS29iALS-C9n1.ISOxx (ISO29), a mutation-corrected isogenic line. A total of 148,223 cells were processed over two batches.
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| Overall design |
Single cell RNA-sequencing data was generated using the 10X Genomics Chromium Single Cell 3' Chip and workflow.
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| |
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| Contributor(s) |
Wenger LM, Szebenyi K, Gibbons GM, Lakatos A |
| Citation(s) |
34675437 |
| Submission date |
Jul 14, 2021 |
| Last update date |
Nov 10, 2021 |
| Contact name |
Lea Marion Denise Wenger |
| E-mail(s) |
lmdw2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Clinical Neurosciences
|
| Lab |
Lakatos
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0PY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA747250 |
| SRA |
SRP328681 |
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