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Series GSE180191 Query DataSets for GSE180191
Status Public on Mar 02, 2022
Title Maelstrom functions in the production of Siwi-piRISC capable of regulating transposons in Bombyx germ cells
Organism Bombyx mori
Experiment type Expression profiling by high throughput sequencing
Other
Summary PIWI-interacting RNAs (piRNAs) bind to PIWI proteins to assemble the piRISC, which represses germline transposons. Maelstrom (Mael) is necessary for piRISC biogenesis in germ cells, but its function remains unclear. Here, we show that Mael interconnects Spindle-E (Spn-E), a key piRISC biogenesis factor, with unloaded Siwi, one of two silkworm PIWI members. Mael also assembles a subset of nuage, a non-membranous organelle involved in piRISC biogenesis. Loss of Mael abrogated the Spn-E–Siwi interaction and Ago3-piRISC biogenesis, but Siwi-piRISC was produced. Bioinformatic analysis showed that Siwi-bound piRNAs in Mael-lacking cells were rich in transposon-targeting piRNAs as in normal cells but were biased toward transposons that are marginally controlled by Siwi-piRISC. This explains the impairment in Ago3-piRISC production because transposon mRNAs cleaved by Siwi are the origin of Ago3-loaded piRNAs. We argue that Mael plays a role in the production of primary Siwi-piRISC capable of regulating transposon expression in germ cells.
 
Overall design Six smRNA-seq libraries: PIWI-associated small RNA libraries were prepared from small RNAs associated with Flag-Siwi immunoprecipitates with size selection at 22-35nt.
Six RNA-seq libraries: RNA libraries were prepared from total RNAs with poly-A selection.
 
Contributor(s) Namba Y, Siomi MC
Citation(s) 35243263
https://0-doi-org.brum.beds.ac.uk/10.1016/j.isci.2022.103914
Submission date Jul 15, 2021
Last update date Mar 18, 2022
Contact name Yurika Namba
E-mail(s) yurika-namba@g.ecc.u-tokyo.ac.jp
Organization name The University of Tokyo
Department Department of Biological Sciences, Graduate School of Science
Lab Siomi Lab
Street address 2-11-16 Yayoi, Bunkyo-ku
City Tokyo
State/province --- Select a state ---
ZIP/Postal code 113-0032
Country Japan
 
Platforms (2)
GPL18754 Illumina MiSeq (Bombyx mori)
GPL29210 Illumina NovaSeq 6000 (Bombyx mori)
Samples (12)
GSM5455094 LucKD_Siwi-piRNA_rep1
GSM5455095 LucKD_Siwi-piRNA_rep2
GSM5455096 Ago3KD_Siwi-piRNA_rep1
Relations
BioProject PRJNA747001
SRA SRP328533

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE180191_RNA-seq_processed.txt.gz 54.8 Kb (ftp)(http) TXT
GSE180191_Transposons_Namba2021.fasta.gz 510.2 Kb (ftp)(http) FASTA
GSE180191_smRNA-seq_processed.txt.gz 46.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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