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| Status |
Public on Sep 11, 2009 |
| Title |
An Estrogen Receptor α-bound Human Chromatin Interactome |
| Organism |
Homo sapiens |
| Experiment type |
Other
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We developed Chromatin Interaction Analysis by Paired-End Tag sequencing (ChIA-PET) for de novo detection of global chromatin interactions, and comprehensively mapped the chromatin interaction network bound by estrogen receptor α (ERα) in the human genome. We performed 454 and Illumina sequencing analyses.
Keywords: Epigenetics
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| Overall design |
Using 454, we examined 3 libraries: IHM001 (Estrogen Receptor ChIA-PET), IHM043 (Estrogen Receptor ChIP-PET) and IHM062 (IgG ChIA-PET)
Using Illumina, we examined 4 libraries: IHM001 (Estrogen Receptor ChIA-PET replicate 1, Paired End Sequencing), IHH015 (Estrogen Receptor ChIA-PET replicate 2, Paired End Sequencing), H3K4me3 ChIP-Seq and RNA polymerase II ChIP-Seq
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| |
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| Contributor(s) |
Ruan Y, Fullwood M |
| Citation(s) |
19890323, 20181287 |
| Submission date |
Sep 09, 2009 |
| Last update date |
Mar 19, 2020 |
| Contact name |
Yijun Ruan |
| E-mail(s) |
ruanyj@gis.a-star.edu.sg
|
| Phone |
65 6478 8073
|
| URL |
http://www.gis.a-star.edu.sg
|
| Organization name |
Genome Institute of Singapore
|
| Department |
Research Computing Group
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| Street address |
60 Biopolis Street, #02-01 Genome, Singapore 138672
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| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (3) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL9178 |
454 GS 20 (Homo sapiens) |
| GPL9186 |
454 GS FLX (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA119301 |
| SRA |
SRP001979 |
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