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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 07, 2021 |
| Title |
Immediate Early Response 2 (Ier2) overexpression effect in NIH 3T3 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of Immediate Early Response 2 (Ier2)-inducible NIH 3T3 cells after Ier2 induction with RheoSwitch ligand RSL-1. Results provide insight into the function of Ier2 in NIH 3T3 mouse embryonal fibroblasts.
Immediate early genes, including Ier2, are rapidly induced in quiescent cells by proliferation and migration-inducing stimuli. Microarray gene expression profiling was performed to identify differentially expressed genes following overexpression of Ier2 in NIH 3T3-Ier2 inducible cells after 24 hour induction of Ier2.
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| Overall design |
To prepare inducible NIH 3T3-RheoSwitch-Ier2 cells, NIH 3T3 cells were transfected with pNEBR-Ier2-hygro and pNEBR-R1 plasmids (Wallbaum et al., 2009, Biotechniques, 46: 433-440; PMID: 19480637). To induce Ier2 expression, NIH 3T3-RheoSwitch-Ier2 cells were treated with RheoSwitch ligand RSL-1.
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| Contributor(s) |
Sleeman JP, Wallbaum S |
| Citation(s) |
34611309 |
| Submission date |
Jul 30, 2021 |
| Last update date |
Oct 09, 2021 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
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| Department |
ZMF
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| Street address |
Theodor-Kutzer-Ufer
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| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
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| Platforms (1) |
| GPL7546 |
Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mm_ENTREZG_10] |
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| Samples (12)
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| GSM5492337 |
NIH 3T3-RheoSwitch-Ier2, DMSO, biological rep1 |
| GSM5492338 |
NIH 3T3-RheoSwitch-Ier2, DMSO, biological rep2 |
| GSM5492339 |
NIH 3T3-RheoSwitch-Ier2, DMSO, biological rep3 |
| GSM5492340 |
NIH 3T3-RheoSwitch-Ier2, RSL-1, biological rep1 |
| GSM5492341 |
NIH 3T3-RheoSwitch-Ier2, RSL-1, biological rep2 |
| GSM5492342 |
NIH 3T3-RheoSwitch-Ier2, RSL-1, biological rep3 |
| GSM5492343 |
NIH 3T3, DMSO, biological rep1 |
| GSM5492344 |
NIH 3T3, DMSO, biological rep2 |
| GSM5492345 |
NIH 3T3, DMSO, biological rep3 |
| GSM5492346 |
NIH 3T3, RSL-1, biological rep1 |
| GSM5492347 |
NIH 3T3, RSL-1, biological rep2 |
| GSM5492348 |
NIH 3T3, RSL-1, biological rep3 |
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| Relations |
| BioProject |
PRJNA751048 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE181200_RAW.tar |
75.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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