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| Status |
Public on Nov 30, 2021 |
| Title |
MicroRNA profiling of Gingival tissue from chronically SIV infected rhesus macaques |
| Platform organism |
Homo sapiens |
| Sample organism |
Macaca mulatta |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
The study describes miRNA expression in Gingival tissue of chronically SIV-infected rhesus macaques. HIV/SIV-associated periodontal disease (gingivitis/periodontitis) (PD) represents a major comorbidity affecting HIV patients on anti-retroviral therapy. Employing a systems biology approach, we report molecular changes underlying PD and its modulation by phytocannabinoids [delta-9-tetrahydrocannabinol (9-THC)] in uninfected and SIV-infected rhesus macaques (RMs) untreated (VEH-untreated/SIV) or treated with vehicle (VEH/SIV) or 9-THC (THC/SIV). VEH- untreated/SIV but not THC/SIV RMs showed significant enrichment of genes linked to anti-viral defense, interferon-beta, NFkB, RIG-1, and JAK-STAT signaling. We focused on the anti-microbial DUOX1 and immune activation marker IDO1 that were reciprocally regulated in gingiva of VEH-untreated/SIV RMs. Both proteins localized to the gingival epithelium and CD163+ macrophages, and showed differential expression in the gingiva of THC/SIV and VEH/SIV RMs. Additionally, inflammation-associated miR-21, miR-142-3p, miR-223, and miR-125a-5p showed significantly higher expression in the gingiva of VEH/SIV RMs. In human primary gingival epithelial cells, miR-125a-5p post-transcriptionally downregulated DUOX1 These findings strongly support a role for differential miRNA expression associated with HIV/SIV induced gingival mucosal dysfunction.
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| Overall design |
Thirteen age and weight matched male Indian rhesus macaques were randomly divided into 3 groups. Group 1 (n=4) remained uninfected. Group 2 (VEH/SIV, n=6) animals were infected intravenously with 100TCID50 of SIVmac251. Group 3 (THC/SIV, n=3) were treated with delta-9-tetrahydrocannabinol and infected intravenously with 100TCID50 SIVmac251. Gingival tissue was collected at necropsy, ~180 days post SIV infection. ~100 ng of total RNA was first reverse transcribed and preamplified according to the manufacturer’s recommendation. MicroRNA expression profiling was performed using TaqMan ®OpenArray® Human microRNA panels. Data analysis was performed using ExpressionSuite® software, which utilizes the comparative Cτ (ΔΔCτ) method to rapidly and accurately quantify relative miRNA expression across many genes and samples. miRNA expression data were normalized using global normalization method. Comparisons were made between uninfected and VEH/SIV and THC/SIV and VEH/SIV rhesus macaques.
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| Contributor(s) |
Mohan M |
| Citation(s) |
34954656 |
| Submission date |
Aug 05, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Mahesh Mohan |
| E-mail(s) |
mmohan@txbiomed.org
|
| Organization name |
Southwest National Primate Research Center
|
| Department |
Host Pathogen Interaction Program
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| Lab |
12/106
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| Street address |
8715 West Military Road
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78227 |
| Country |
USA |
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| Platforms (1) |
| GPL17837 |
TaqMan OpenArray Human MicroRNA Panel (4461104) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA752386 |
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