|
| Status |
Public on Apr 16, 2022 |
| Title |
Diurnal oscillations in murine brown adipose tissue analyzed by RNA-seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Brown adipose tissue (BAT) burns fatty acids to produce heat, and shows diurnal oscillation with peak activity in glucose and triglyceride-derived fatty acid uptake at wakening. To gain further insight in the diurnal regulation of metabolic BAT activity, we performed RNA-sequencing (RNA-seq) on interscapular (i)BAT samples collected from chow-fed male C57BL/6J mice (10 weeks old) exposed to mild cold (22°C) at 3-hour intervals throughout a 24-hour period (n=4 mice per time point). All mice were entrained to a 12h:12h light:dark cycle, and therefore time is denoted as Zeitgeber Time (ZT) in which ZT0 indicates the onset of the light (active) phase.
|
| |
|
| Overall design |
We applied RNA-seq on total RNA isolated from murine iBAT obtained at 3 hour-intervals throughout a 24-hour period (8 time points in total; 4 replicates per time point).
|
| |
|
| Contributor(s) |
van Eenige R, In het Panhuis W, Rensen PC, Kooijman S |
| Citation(s) |
35413480 |
| Submission date |
Aug 13, 2021 |
| Last update date |
Apr 17, 2022 |
| Contact name |
Robin van Eenige |
| Organization name |
Leiden University Medical Center
|
| Department |
Division of Endocrinology, Department of Medicine
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| ZIP/Postal code |
2333 ZA |
| Country |
Netherlands |
| |
|
| Platforms (1) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA754362 |
| SRA |
SRP332432 |
Less...
More...