|
| Status |
Public on Aug 05, 2022 |
| Title |
TLR7 sensing of SARS-CoV-2 and IFN-I response by plasmacytoid dendritic cells leads to the hyperactivation of macrophages. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To gain a comprehensive understanding of type I IFN or SARS-CoV-2 activated pDCs priming macrophages in regulating TLR4 meidated gene expression, we treated primary human blood monocytes with 30ng/ml IFNα or the supernatant of SARS-CoV-2 activated pDCs for 24 h and followed by 2 ng/ml LPS for 3 h. Cells were harvested and performed transcriptomic analysis via RNA-seq. We found that IFNα and supernatant of SARS-CoV-2 activated pDCs had similar effects on priming macrophages to induce massive inflammatory gene expression mediated by LPS.
|
| |
|
| Overall design |
mRNA of human primary monoyctes from 4 healthy donors treated with 30ng/ml IFNα or the supernatant of SARS-CoV-2 activated pDCs for 24 h and followed by 2 ng/ml LPS for 3 h were used for library preparing and RNA sequencing.
|
| |
|
| Contributor(s) |
Yang C, Oliver DJ, Barrat FJ |
| Citation(s) |
36083891 |
| Submission date |
Aug 23, 2021 |
| Last update date |
Nov 16, 2022 |
| Contact name |
HSS Genomics Research Center |
| Organization name |
Hospital for Special Surgery
|
| Department |
Research
|
| Street address |
535 E 70th St
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA756948 |
| SRA |
SRP333686 |
Less...
More...