 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 10, 2009 |
| Title |
Expression data for kidney progenitor tissue from rat embryo at E13 |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study we compared genes expressed in the unbudded portion of the Wolffian duct with the isolated ureteric bud to find genes novel to early kidney development. We used the Affymetrix Rat Genome 230 2.0 Array to compare the unbudded tissues with the budded samples.
We used microarrays to find novel genes regulating early kidney formation.
|
| |
|
| Overall design |
Isolated ureteric buds (UB) were separated from the embryonic kidney after a light trypsinization step. Approximately 100 UBs were pooled together into each replicate. Approximately 100 isolated Wolffian ducts were dissected from E13 rat embryoes and pooled into each sample. Approximately 50 Wolffian ducts with attached mesodermal cells were pooled into each replicate.
|
| |
|
| Contributor(s) |
Choi Y, Tee JB, Kitamura S, Nigam SK |
| Citation(s) |
19934016 |
| Submission date |
Sep 24, 2009 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Yohan Choi |
| Organization name |
UCSD
|
| Street address |
9500 Gilman Drive, 0693
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
|
| Samples (9)
|
| GSM456312 |
embryo at E13 WD, biological rep1 |
| GSM456313 |
embryo at E13 WD, biological rep2 |
| GSM456314 |
embryo at E13 WD, biological rep3 |
| GSM456315 |
embryo at E13 WD+IM, biological rep1 |
| GSM456316 |
embryo at E13 WD+IM, biological rep2 |
| GSM456317 |
embryo at E13 WD+IM, biological rep3 |
|
| Relations |
| BioProject |
PRJNA117931 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18260_RAW.tar |
24.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...