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| Status |
Public on Aug 29, 2021 |
| Title |
microRNA profile of control vehicle- and coagulation protease factor VIIa-released endothelial extracellular vesicles |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Coagulation protease factor VIIa (FVIIa) is shown to induce anti-inflammatory and barrier protective effects via endothelial cell protein C receptor (EPCR)-dependent, protease-activated receptor-1 (PAR1)-mediated cell signaling. FVII-EPCR-PAR1 signaling also induces the release of extracellular vesicles from endothelial cells. To obtain clues on whether microRNA (miR) carried out by FVIIa-released EEVs contribute to anti-inflammatory and barrier protective effects, we analyzed miR expression in control- and FVIIa-released EEVs by deep sequencing. These data revealed that several anti-inflammatory miR expression was higher (more than 2-fold) in FVIIa-released EEVs compared to control EEVs, the most predominant being miR10a-5p. The differential expression of miR10a-5p and several other abundant miRs were validated by qRT-PCR. Subsequent in vitro and in vivo experiments showed that miR10a in FVIIa-released EEVs contribute to anti-inflammatory and barrier protective effects.
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| Overall design |
EEVs were isolated from control vehicle- and FVIIa-treated human umbilical vein endothelial cells (24 h treatment). Equal number of EEVs (1x109) were subjected to microRNA analysis by deep-sequencing (UT Southwestern Core Facility)
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| Contributor(s) |
Das K, Mohan Rao LV, Pendurthi U |
| Citation(s) |
34469511 |
| Submission date |
Aug 27, 2021 |
| Last update date |
Dec 17, 2021 |
| Contact name |
Chengsong Zhu |
| E-mail(s) |
chengsongzhu@gmail.com
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| Phone |
2146455160
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| Organization name |
UT Southwestern Medical Center
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| Department |
Immunology
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| Lab |
Microarray & Immune Phenotyping Core
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| Street address |
5323 Harry Hines Blvd
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| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA758366 |
| SRA |
SRP334460 |
