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| Status |
Public on Sep 24, 2021 |
| Title |
Human resident memory T cells exit the skin and mediate systemic Th2-driven inflammation. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the transcriptional profile of human circulating T cells with skin tissue-resident memory phenotype (cTRM) in GVHD we performed bulk RNA sequencing from SMART-seq2 of sorted human cTRM (CD3+ CD4+ CLA+ CD103+) and conventional T cells (CD3+ CD4+ CD103-), and single cell RNA sequencing from 10X Genomics of sorted human CD45+, both from isolated blood PBMCs of patients with active acute GVHD.
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| Overall design |
Frozen PBMC isolated of whole blood from patients with active acute GVHD were thawed, stained for FACS-sorting and sorted for live CD45+ cells, which were submitted for 10X Genomics single-cell RNA sequencing (n=2, two replicates for donor 2), or sorted for live cTRM (live CD45+ CD3+ CD4+ CLA+ CD103) and Tconv (live CD45+ CD3+ CD4+ CD103-), wich were submitted for SMART-seq2 bulk RNA sequencing (n=3).
*** Submitter Comment: Due to security issue we are not submitting the raw data ***
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| Contributor(s) |
Strobl J, Gail LM, Dingelmaier-Hovorka R, Krausgruber T, Pandey RV, Stary G |
| Citation(s) |
34643646 |
| Submission date |
Sep 01, 2021 |
| Last update date |
Dec 24, 2021 |
| Contact name |
Georg Stary |
| Organization name |
Medical University of Vienna
|
| Department |
Dermatology
|
| Street address |
Spitalgasse 23
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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| GSM5553351 |
Tconv_Donor1: Conventational T cells donor 1 |
| GSM5553352 |
Tconv_Donor2: Conventational T cells donor 2 |
| GSM5553353 |
Tconv_Donor3: Conventational T cells donor 3 |
| GSM5553354 |
cTRM_Donor1: Circulating tissue resident memory T cells donor 1 |
| GSM5553355 |
cTRM_Donor2: Circulating tissue resident memory T cells donor 2 |
| GSM5553356 |
cTRM_Donor3: Circulating tissue resident memory T cells donor 3 |
| GSM5553357 |
TK281s1: GVHD donor1 |
| GSM5553358 |
TK212s1: GVHD donor2 |
| GSM5553359 |
TK212s2: GVHD donor2 |
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| Relations |
| BioProject |
PRJNA759621 |
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