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| Status |
Public on Oct 19, 2021 |
| Title |
RNA-seq analysis of HeLa cells following MYC inhibition with ASO |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed RNA-seq on HeLa cells treated with MYCASO-3, a non-targeting ASO (NT-ASO), Lipofectamine alone, and untreated cells
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| |
|
| Overall design |
Cells were treated for 4h or 18h with 10 nM ASO (formulated with Lipofectamine 2000 reagent). RNA was extracted with the QIAGEN RNeasy Kit, followed by library preparation and sequencing.
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| |
|
| Contributor(s) |
Ott CJ |
| Citation(s) |
34650218 |
| Submission date |
Sep 06, 2021 |
| Last update date |
Apr 12, 2022 |
| Contact name |
Christopher J Ott |
| E-mail(s) |
Christopher.Ott@MGH.HARVARD.EDU
|
| Phone |
6176435895
|
| Organization name |
Mass Gen Hospital/Harvard Medical School
|
| Street address |
149 13th Street, Rm 6614
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (21)
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|
| Relations |
| BioProject |
PRJNA761147 |
| SRA |
SRP335902 |
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