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| Status |
Public on Sep 09, 2021 |
| Title |
Repeated exposure with short-term behavioral stress resolves pre-existing stress-induced depressive-like behavior in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Chronic stress induces adaptive changes in the brain via the cumulative action of glucocorticoids, which is associated with mood disorders. Here we show that repeated daily five-minute restraint resolves pre-existing stress-induced depressive-like behavior in mice. Repeated injection of glucocorticoids in low doses mimics the anti-depressive effects of short-term stress. Repeated exposure to short-term stress and injection of glucocorticoids activate neurons in largely overlapping regions of the brain, as shown by c-Fos staining, and reverse distinct stress-induced gene expression profiles. Chemogenetic inhibition of neurons in the prelimbic cortex projecting to the nucleus accumbens, basolateral amygdala, or bed nucleus of the stria terminalis results in anti-depressive effects similarly to short-term stress exposure, while only inhibition of neurons in the prelimbic cortex projecting to the bed nucleus of the stria terminalis rescues glucocorticoid release. In summary, we show that short-term stress can reverse adaptively altered stress gains and resolve stress-induced depressive-like behavior.
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| Overall design |
Two independent sets of control (CON), CRST, and CRST+RS5 groups were prepared. Mice were subjected to CRST treatment followed by RS5, and then sacrificed on post-stress day 22. Total RNA was extracted from the pooled PL tissue of 6 – 7 animals in each group. RNA extraction and hybridization on Illumina microarrays.
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| Contributor(s) |
Lee E |
| Citation(s) |
34795225 |
| Submission date |
Sep 07, 2021 |
| Last update date |
Dec 10, 2021 |
| Contact name |
Eun-Hwa Lee |
| E-mail(s) |
gonobplaypan@gmail.com
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| Organization name |
Ewha Womans University
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| Street address |
Science building B214, 52 Ewhayeodae-gil, Seodaemun-gu
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| City |
Seoul |
| ZIP/Postal code |
03760 |
| Country |
South Korea |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (6)
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| GSM5563665 |
Chronic restraint stress 2 h for 14 days rep2 |
| GSM5563666 |
Chronic restraint stress 2 h for 14 days and then chronic short-term restraint stress for 5 min for 14 days rep 1 |
| GSM5563667 |
Chronic restraint stress 2 h for 14 days and then chronic short-term restraint stress for 5 min for 14 days rep 2 |
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| Relations |
| BioProject |
PRJNA761420 |
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