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| Status |
Public on Jun 15, 2022 |
| Title |
The long non-coding RNA mimi scaffolds neuronal granules to maintain nervous system maturity |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-binding proteins and messenger RNAs assemble into ribonucleoprotein granules that regulate mRNA trafficking, local translation, and turnover. The dysregulation of RNA-protein condensation disturbs synaptic plasticity and neuron survival, and has been widely associated with human neurological disease. Neuronal granules are thought to condense around particular proteins that dictate the identity and composition of each granule type. Here, we show in Drosophila that a previously uncharacterized long non-coding RNA, mimi, is required to scaffold large neuronal granules in the adult nervous system. Neuronal ELAV-like proteins directly bind mimi and mediate granule assembly, while Staufen maintains condensate integrity. mimi granules contain mRNAs and proteins involved in synaptic processes; granule loss in mimi mutant flies impairs nervous system maturity, neuropeptide-mediated signaling and causes phenotypes of neurodegeneration. Our work reports the first architectural RNA for a neuronal granule and provides a handle to interrogate functions of a condensate independently from those of its constituent proteins.
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| Overall design |
totalRNAseq of FNE, RBP9 mutants and wild-type control. 5 replicates per genotype; 4 different genotypes: wild type (w1118), Δfne (fneΔ/fneKOZ2 (females) and fneΔ or fneKOZ2 (males)), Δrbp9 (rbp9P2690/Df(2L)ED206 (males and females)) and ΔfneΔrbp9 (fneΔ/fneKOZ2; rbp9P2690/Df(2L)ED206 (females) and fneΔ; rbp9P2690/ Df(2L)ED206 or fneKOZ2; rbp9P2690/Df(2L)ED206 (males))
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| Contributor(s) |
Grzejda D, Mach J, Schweizer J, Hummel B, Rezansoff AM, Eggenhofer F, Panhale A, Lalioti M, Cabezas Wallscheid N, Backofen R, Felsenberg J, Hilgers V |
| Citation(s) |
36170367 |
| Submission date |
Sep 09, 2021 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Valérie Hilgers |
| Organization name |
MPI of Immunobiology and Epigenetics
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| Street address |
Stübeweg 51
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| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE183816 |
The long non-coding RNA mimi scaffolds neuronal granules to maintain nervous system maturity |
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| Relations |
| BioProject |
PRJNA762048 |
| SRA |
SRP336388 |
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