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| Status |
Public on Sep 16, 2021 |
| Title |
Rapid depletion of the cohesin subunits and CTCF reveals their role in maintaining high-order genome architecture [DamID and ChIP] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Cohesin stalling at CTCF binding sites represents one of the main principles of interphase chromosome organization. In the current studies, we dissect the role of cohesin and CTCF, both alone and in combination, in 3D genome organization by depleting these proteins using acute protein degradation techniques. By systematic examination of interactomic, epigenomic and transcriptomic changes using various sequencing techniques, our studies reveal the functions of cohesin and CTCF in mediating the formation of chromatin loops, topologically associating domains, chromosome compartments and nuclear lamina associating domains. Our studies describe fundamental principles of how the architectural proteins contribute to genome folding at multiple genomic scales and transcriptional regulation.
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| Overall design |
Data files generated to determine CTCF enrichment at the borders of lamina-associated domains (LADs) in mouse neural precursor cells (mNPCs). H3K27me3 ChIP-seq data files from a CTCF depletion time course in mouse embryonic stem cells (mESCs) witih AID-tagged CTCF.
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| Contributor(s) |
van Schaik T, Liu NQ, de Wit E, van Steensel B |
| Citation missing |
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| Submission date |
Sep 12, 2021 |
| Last update date |
Feb 17, 2022 |
| Contact name |
Bas van Steensel |
| Organization name |
Netherlands Cancer Institute
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| Department |
Gene Regulation
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| Street address |
Plesmanlaan 121
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| City |
Amsterdam |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA762533 |
| SRA |
SRP336713 |
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