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| Status |
Public on Sep 15, 2021 |
| Title |
RNA-seq of limmmunocompetent mice in response to Aspergillus fumigatus conidia and hyphae |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We bulit a pulmonary aspergillosis model in immunocompetent mice. Mice were inoculated intratracheally with 50 μL suspension of conidia , hyphal fragments or normal saline, respectively, after anesthesia. Lungs from were harvested and ground for RNA extraction 24 hours after fungal inoculation. The result of RNA-seq indicated both conidia and hyphae can activate a series of immunologically relevant pathways and these pathways were much more strongly activated by hyphae. Hyphae could promote Th2 cell differentiation and suppress Th1 cell differentiation. Both hyphae and conidia could activate Th17 cell differentiation.
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| Overall design |
RNA profile of pulmonary aspergillosis in response to different states of A. fumigatus
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| Contributor(s) |
Luo Y, deng L, Liu F, Xu J, Kong Q, Shi Y, Sang H |
| Citation missing |
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| Submission date |
Sep 12, 2021 |
| Last update date |
Sep 18, 2021 |
| Contact name |
Yingzhi Luo |
| Organization name |
Jinling Hospital
|
| Street address |
Meiyuan street
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| City |
Nanjing |
| ZIP/Postal code |
210018 |
| Country |
China |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA762564 |
| SRA |
SRP336738 |
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