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Series GSE184194 Query DataSets for GSE184194
Status Public on Sep 16, 2021
Title Placental exosomes polarize human monocyte-derived macrophages to a decidual M2-like macrophage phenotype
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Macrophages are abundant in uterine mucosa during the peri-implantation phase and early pregnancy. Decidual macrophages display dynamic changes alone with pregnancy progress: During the peri-implantation phase, macrophages displayed a pro-inflammatory phenotype which facilitates embryo implantation. While, In the late firster trimester and second trimester, decidual macrophages are anti-proinflammatory which are helpful to pregnancy maintenance. Alterations in the ratio of pro-inflammatory/anti-inflammatory decidual macrophages lead to abortion, preeclampsia, and preterm birth. Placenta-derived exosomes (pEXO) are critical in the immune cell modulation such as T cell apoptosis, NK activities, and T regulatory (Treg) differentiation. However, it is unknown whether placenta-derived exosomes contribute to decidual macrophage polarization during early pregnancy. Here we report that exosomes from the placenta explant stimulate M2 macrophage polarization via exosomal miRNA-30d-5p. Mechanistically, miRNA-30d-5p polarized macrophages to M2 phenotype by inhibiting HDAC9 expression. Furthermore, the conditioned medium of pEXO-treated macrophages promoted trophoblast migration and invasion. By contrast, conditioned medium impaired the ability of endothelial cell tube formation. However, pEXO-treated macrophages have no impact on T cell proliferation. Together, we demonstrated that pEXO promoted trophoblast migration and invasion, endothelial cell migration, and attenuation of endothelial cell tube formation by polarizing macrophage to decidua-like macrophage.
Overall design mRNA profiles of pEXO-treated macrophages (N=2) and Control macrophage (N=2)
Contributor(s) Chiu PC, Kunfeng B
Citation(s) 35180876
Submission date Sep 15, 2021
Last update date Feb 28, 2022
Contact name Jianlin Li
Organization name The university of Hong Kong
Street address L 7-08, Department of Obstetrics and Gynecology Laboratory block, 21th Sassoon Road, Hong Kong
City Hong Kong
ZIP/Postal code 999077
Country Hong Kong
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM5580099 Contorl_macrophage_01
GSM5580100 Contorl_macrophage_02
GSM5580101 pEXO_macrophage_01
This SubSeries is part of SuperSeries:
GSE184195 Placental exosomes
BioProject PRJNA763535
SRA SRP337239

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184194_Normalized_gene_counts_matrix.txt.gz 374.8 Kb (ftp)(http) TXT
GSE184194_Raw_gene_counts_matrix.txt.gz 197.2 Kb (ftp)(http) TXT
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