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| Status |
Public on Sep 16, 2021 |
| Title |
Placental exosomes polarize human monocyte-derived macrophages to a decidual M2-like macrophage phenotype |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophages are abundant in uterine mucosa during the peri-implantation phase and early pregnancy. Decidual macrophages display dynamic changes alone with pregnancy progress: During the peri-implantation phase, macrophages displayed a pro-inflammatory phenotype which facilitates embryo implantation. While, In the late firster trimester and second trimester, decidual macrophages are anti-proinflammatory which are helpful to pregnancy maintenance. Alterations in the ratio of pro-inflammatory/anti-inflammatory decidual macrophages lead to abortion, preeclampsia, and preterm birth. Placenta-derived exosomes (pEXO) are critical in the immune cell modulation such as T cell apoptosis, NK activities, and T regulatory (Treg) differentiation. However, it is unknown whether placenta-derived exosomes contribute to decidual macrophage polarization during early pregnancy. Here we report that exosomes from the placenta explant stimulate M2 macrophage polarization via exosomal miRNA-30d-5p. Mechanistically, miRNA-30d-5p polarized macrophages to M2 phenotype by inhibiting HDAC9 expression. Furthermore, the conditioned medium of pEXO-treated macrophages promoted trophoblast migration and invasion. By contrast, conditioned medium impaired the ability of endothelial cell tube formation. However, pEXO-treated macrophages have no impact on T cell proliferation. Together, we demonstrated that pEXO promoted trophoblast migration and invasion, endothelial cell migration, and attenuation of endothelial cell tube formation by polarizing macrophage to decidua-like macrophage.
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| Overall design |
mRNA profiles of pEXO-treated macrophages (N=2) and Control macrophage (N=2)
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| Contributor(s) |
Chiu PC, Kunfeng B |
| Citation(s) |
35180876 |
| Submission date |
Sep 15, 2021 |
| Last update date |
Feb 28, 2022 |
| Contact name |
Jianlin Li |
| Organization name |
The university of Hong Kong
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| Street address |
L 7-08, Department of Obstetrics and Gynecology Laboratory block, 21th Sassoon Road, Hong Kong
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| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA763535 |
| SRA |
SRP337239 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE184194_Normalized_gene_counts_matrix.txt.gz |
374.8 Kb |
(ftp)(http) |
TXT |
| GSE184194_Raw_gene_counts_matrix.txt.gz |
197.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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