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Series GSE184194 Query DataSets for GSE184194
Status Public on Sep 16, 2021
Title Placental exosomes polarize human monocyte-derived macrophages to a decidual M2-like macrophage phenotype
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Macrophages are abundant in uterine mucosa during the peri-implantation phase and early pregnancy. Decidual macrophages display dynamic changes alone with pregnancy progress: During the peri-implantation phase, macrophages displayed a pro-inflammatory phenotype which facilitates embryo implantation. While, In the late firster trimester and second trimester, decidual macrophages are anti-proinflammatory which are helpful to pregnancy maintenance. Alterations in the ratio of pro-inflammatory/anti-inflammatory decidual macrophages lead to abortion, preeclampsia, and preterm birth. Placenta-derived exosomes (pEXO) are critical in the immune cell modulation such as T cell apoptosis, NK activities, and T regulatory (Treg) differentiation. However, it is unknown whether placenta-derived exosomes contribute to decidual macrophage polarization during early pregnancy. Here we report that exosomes from the placenta explant stimulate M2 macrophage polarization via exosomal miRNA-30d-5p. Mechanistically, miRNA-30d-5p polarized macrophages to M2 phenotype by inhibiting HDAC9 expression. Furthermore, the conditioned medium of pEXO-treated macrophages promoted trophoblast migration and invasion. By contrast, conditioned medium impaired the ability of endothelial cell tube formation. However, pEXO-treated macrophages have no impact on T cell proliferation. Together, we demonstrated that pEXO promoted trophoblast migration and invasion, endothelial cell migration, and attenuation of endothelial cell tube formation by polarizing macrophage to decidua-like macrophage.
 
Overall design mRNA profiles of pEXO-treated macrophages (N=2) and Control macrophage (N=2)
 
Contributor(s) Chiu PC, Kunfeng B
Citation(s) 35180876
Submission date Sep 15, 2021
Last update date Feb 28, 2022
Contact name Jianlin Li
Organization name The university of Hong Kong
Street address L 7-08, Department of Obstetrics and Gynecology Laboratory block, 21th Sassoon Road, Hong Kong
City Hong Kong
ZIP/Postal code 999077
Country Hong Kong
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM5580099 Contorl_macrophage_01
GSM5580100 Contorl_macrophage_02
GSM5580101 pEXO_macrophage_01
This SubSeries is part of SuperSeries:
GSE184195 Placental exosomes
Relations
BioProject PRJNA763535
SRA SRP337239

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184194_Normalized_gene_counts_matrix.txt.gz 374.8 Kb (ftp)(http) TXT
GSE184194_Raw_gene_counts_matrix.txt.gz 197.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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