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Series GSE184621 Query DataSets for GSE184621
Status Public on Sep 24, 2021
Title RNA expression and m5c modification in NSUN2 kncok down CaSki cell
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary 5-methylcytosine (m5C) modification ubiquitously occurs on mammalian mRNAs and plays important roles in multiple biological processes. Currently, the role of m5C in cancer initiation and progression is gaining more and more research attention, but the detailed mechanism remains unclear. As an m5C methyltransferase with unique mRNA catalytic activity, NSUN2 was found to be highly expressed in multiple cancers including gastric, bladder, gallbladder, and breast cancers, suggesting NSUN2 might exert oncogenic properties through affecting m5C levels in cancer cells. Therefore, to understand the potential roles of RNA m5C modification in tumorigenesis of cervical cancer, we established NSUN2 stable knockdown CaSki cell, and perform RNA-seq and RNA-BisSeq to figure out the possible pathway involved in cervical cancer development.
 
Overall design NSUN2 stable knockdown CaSki cell was established by lentivirus infection. RNA of wildtype cells and NSUN2 stable knockdown CaSki cells were extracted for RNA-seq and RNA bisulfite sequencing measurement.
 
Contributor(s) Wang L, Zhang J, Su Y, Hsu C
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Submission date Sep 22, 2021
Last update date Sep 24, 2021
Contact name Lingfang Wang
E-mail(s) 3110103748@zju.edu.cn
Organization name Zhejiang University
Department Department of Obstetrics and Gynecology
Lab Research Building of Zhejiang University School of Medicine C241
Street address 866 Yuhangtang Road
City Hangzhou
State/province Zhejiang Province
ZIP/Postal code 310006
Country China
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM5593825 Sh-3
GSM5593826 ShNC
GSM5593827 Sh-3.m5C
Relations
BioProject PRJNA765374
SRA SRP338261

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Supplementary file Size Download File type/resource
GSE184621_Gene_Expression_Profiling.xlsx 4.3 Mb (ftp)(http) XLSX
GSE184621_Methylated_RNA_sites.mRNA.xlsx 3.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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