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Series GSE18463 Query DataSets for GSE18463
Status Public on Oct 01, 2013
Title The effects of Hmgb1 depletion on gene expression in murine cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary High Mobility Group Box 1 (HMGB1) is a highly abundant and evolutionarily conserved non-histone chromatin component with the ability to bind and bend DNA. The protein is involved in fundamental nuclear processes including nucleosome sliding, transcription, replication, V(D)J recombination and DNA transposition. To assess the functional impact of HMGB1 on transcription we performed gene expression profile analyses of mouse embryonic fibroblasts, wild-type or knockout for the Hmgb1 gene
Overall design 2 genetic backgrounds: wild-type (Wt), Hmgb1-/- (KO), 3 biological replicates (rep1, rep2, rep3; S phase synchronized cells in duplicate only), no technical replicates. Each pair of wt and Hmgb1-/- MEFs was isolated from embryos born from a single Hmgb1+/- mother crossed with a Hmgb1 +/- male. Total RNAs from three pairs of MEFs, derived from three different mothers and cultured up to passage 3, were extracted using mirVana miRNA Isolation Kit (Ambion) and analyzed on the Illumina BeadsArray platform.
Contributor(s) Celona B, Agresti A, Bianchi ME, Rossetti G, Rossi RL, Pagani M
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Submission date Oct 07, 2009
Last update date Jun 14, 2018
Contact name Riccardo Lorenzo Rossi
Organization name Fondazione INGM
Department Integrative Biology
Lab Pagani
Street address Via Trivulzio 10
City Milano
State/province MI
ZIP/Postal code 20149
Country Italy
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM459866 Wt_cycling_rep3
GSM459867 Wt_cycling_rep1
GSM459868 Wt_cycling_rep2
BioProject PRJNA118187

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18463_RAW.tar 3.1 Mb (http)(custom) TAR
GSE18463_non-normalized.txt.gz 2.5 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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