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Series GSE184844 Query DataSets for GSE184844
Status Public on Oct 12, 2021
Title Calcitriol ameliorates damage in high-salt diet-induced hypertension: evidence of communication with the gut–kidney axis
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Several studies have established a link between high-salt diet, inflammation, and hypertension. Vitamin D supplementation has shown anti-inflammatory effects in many diseases; gut microbiota is also associated with a wide variety of cardiovascular diseases, but potential role of vitamin D and gut microbiota in high-salt diet-induced hypertension remains unclear. Therefore, we used rats with hypertension induced by a high-salt diet as the research object and analyzed the transcriptome of their tissues (kidney and colon) and gut microbiome to conduct an overall analysis of the gut–kidney axis. We aimed to confirm the effects of high salt and calcitriol on the gut–kidney immune system and the composition of the intestinal flora. We demonstrate that consumption of a high-salt diet results in hypertension and inflammation in the colon and kidney and alteration of gut microbiota composition and function. High-salt diet-induced hypertension was found to be associated with seven microbial taxa and mainly associated with reduced production of the protective short-chain fatty acid butyrate. Calcitriol can reduce colon and kidney inflammation, and there are gene expression changes consistent with restored intestinal barrier function. The protective effect of calcitriol may be mediated indirectly by immunological properties. Additionally, the molecular pathways of the gut microbiota-mediated BP regulation may be related to circadian rhythm signals, which needs to be further investigated. An innovative association analysis of the microbiota may be a key strategy to understanding the association between gene patterns and host.
 
Overall design mRNA profiles of kidney and colon from three groups (normal control (NC) group, high salt model (HS) group, and high salt diet plus calcitriol supplementation (CAL) group) were generated by RNAseq, with n=4~6, using Illumina NEXTSEQ 500 platform.
 
Contributor(s) Ding R, Xiao Z, Jiang Y, Yang Y, Ji Y, Bao X, Xing K, Zhou X, Zhu S
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Submission date Sep 27, 2021
Last update date Nov 05, 2021
Contact name Ruifeng Ding
E-mail(s) ruifengding@usst.edu.cn
Organization name University of Shanghai for Science and Technology
Street address 516 Jungong Road, Yangpu District
City Shanghai
ZIP/Postal code 200093
Country China
 
Platforms (1)
GPL20084 Illumina NextSeq 500 (Rattus norvegicus)
Samples (31)
GSM5598124 NC_Cortex_1
GSM5598125 NC_Cortex_2
GSM5598126 NC_Cortex_3
Relations
BioProject PRJNA766639
SRA SRP338900

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184844_Raw_gene_counts_matrix.csv.gz 519.1 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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