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| Status |
Public on Oct 02, 2021 |
| Title |
Gene expression profiling in ninaE wild type and ninaE G69D/+ Drosophila photoreceptors |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cell type specific gene expression profiling was performed with adult Drosophila photoreceptors. Two genotypes were compared: ninaE wild type control and ninaEG69D/+ mutants that have chronic endoplasmic reticulum stress. To specifically isolate RNAs from these cell types, we drove nuclear envelope-localized EGFP::Msp300KASH expression with the photoreceptor specific Rh1-Gal4 driver. Anti-GFP beads were used to isolate the nuclei from those adult fly photoreceptor cells. Novaseq was used to perform RNA-seq.
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| Overall design |
For adult photoreceptor purification, we followed a published protocol by Vikki Weake and colleagues (Hall et al., (2017) Transcriptome profiling of aging Drosophila photoreceptors reveals gene expression trends that correlate with visual senescence. BMC Genomics 18(1): p. 894).
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| Contributor(s) |
Huang H, Ryoo HD |
| Citation missing |
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| Submission date |
Sep 30, 2021 |
| Last update date |
Oct 03, 2021 |
| Contact name |
Hyung Don Ryoo |
| E-mail(s) |
ryooh01@nyumc.org
|
| Phone |
2122637257
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| Organization name |
NYU School of Medicine
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| Department |
Cell Biology
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| Lab |
Ryoo lab
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| Street address |
550 First Avenue, MSB 497
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA767700 |
| SRA |
SRP339587 |
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