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Status |
Public on Oct 09, 2021 |
Title |
RNA sequencing of E. coli NCM3722 over the course of glucose to acetate growth transition |
Organism |
Escherichia coli K-12 |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To understand the gene response during the glucose to acetate diauxic transition, we grew E. coli in minimal media with acetate and a small amount of glucose. Cells were collected and RNA was purified at different time points during the growth transition, including pre-shift (growth on glucose), 5 minutes, 15 minutes, 60 minutes and 120 minutes after glucose run-out, and steady state growth on acetate (post-shift).
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Overall design |
Time-course monitoring of E. coli transcriptome by performing 6 RNA-sequencing experiments during glucose to acetate growth transition.
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Contributor(s) |
Balakrishnan R, Cremer J, Hwa T |
Citation(s) |
34928547 |
Submission date |
Oct 06, 2021 |
Last update date |
Jan 08, 2022 |
Contact name |
Rohan Balakrishnan |
E-mail(s) |
rohanbalakr@gmail.com
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Phone |
6144405008
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Organization name |
UCSD
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Department |
Physics
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Lab |
Hwa lab
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Street address |
9500 Gilman drive
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City |
San Diego |
State/province |
Ca |
ZIP/Postal code |
92093 |
Country |
USA |
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Platforms (1) |
GPL24377 |
Illumina HiSeq 4000 (Escherichia coli K-12) |
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Samples (6)
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Relations |
BioProject |
PRJNA769085 |
SRA |
SRP340400 |