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Series GSE185426 Query DataSets for GSE185426
Status Public on Oct 09, 2021
Title RNA sequencing of E. coli NCM3722 over the course of glucose to acetate growth transition
Organism Escherichia coli K-12
Experiment type Expression profiling by high throughput sequencing
Summary To understand the gene response during the glucose to acetate diauxic transition, we grew E. coli in minimal media with acetate and a small amount of glucose. Cells were collected and RNA was purified at different time points during the growth transition, including pre-shift (growth on glucose), 5 minutes, 15 minutes, 60 minutes and 120 minutes after glucose run-out, and steady state growth on acetate (post-shift).
 
Overall design Time-course monitoring of E. coli transcriptome by performing 6 RNA-sequencing experiments during glucose to acetate growth transition.
 
Contributor(s) Balakrishnan R, Cremer J, Hwa T
Citation(s) 34928547
Submission date Oct 06, 2021
Last update date Jan 08, 2022
Contact name Rohan Balakrishnan
E-mail(s) rohanbalakr@gmail.com
Phone 6144405008
Organization name UCSD
Department Physics
Lab Hwa lab
Street address 9500 Gilman drive
City San Diego
State/province Ca
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL24377 Illumina HiSeq 4000 (Escherichia coli K-12)
Samples (6)
GSM5614863 Pre-shift
GSM5614864 5 minutes
GSM5614865 15 minutes
Relations
BioProject PRJNA769085
SRA SRP340400

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185426_compiled_reads.txt.gz 61.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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