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Status |
Public on Oct 09, 2021 |
Title |
miRNA profiling of colitis-associated dysplasia in animals submitted to the AOM/DSS model |
Organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by array
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Summary |
The impact of the antiinflammatory agent 5-aminosalicylic acid (5-ASA) on the risk for colitis-associated colorectal cancer remains controversial. The chemopreventive activity of 5-ASA was evaluated in the Swiss Webster model of azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colitis-associated neoplasia. Mice were injected with AOM (7.4 mg/kg i.p.) and randomized to receive either vehicle or 5-ASA (75mg/kg) for the remainder of the study. Only untreated animals were used in this array. DSS treatment began at 9 weeks of age and continued for 3 cycles. At the time of sacrifice (18 weeks of age), the entire colon and rectum were processed for histopathologic examination and microarray profiling. For information regarding the histopathological analysis, refer to Clapper ML, Gary MA, Coudry RA, Litwin S, Chang WC, Devarajan K, Lubet RA, Cooper HS. 5-aminosalicylic acid inhibits colitis-associated colorectal dysplasias in the mouse model of azoxymethane/dextran sulfate sodium-induced colitis. Inflamm Bowel Dis. 2008 Oct;14(10):1341-7. doi: 10.1002/ibd.20489. PMID: 18452197.
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Overall design |
Mice were injected with AOM (7.4 mg/kg i.p.) and randomized to receive either vehicle or 5-ASA (75 mg/kg) for the remainder of the study. DSS treatment began at 9 weeks of age and continued for 3 cycles. At the time of sacrifice (18 weeks of age), the entire colon and rectum were processed for histopathologic examination.
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Contributor(s) |
Fragoso MF, Fernandez GJ, Vanderveer L, Cooper H, Slifker M, Clapper M |
Citation |
https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232113024
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Submission date |
Oct 07, 2021 |
Last update date |
Oct 28, 2022 |
Contact name |
Mariana Franco Fragoso |
E-mail(s) |
marifragoso@gmail.com, mariana.fragoso@fccc.edu
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Organization name |
Fox Chase Cancer Center
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Department |
Cancer Prevention and Control
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Street address |
333 Cottman Avenue
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City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19111 |
Country |
USA |
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Platforms (1) |
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Samples (11)
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Relations |
BioProject |
PRJNA769306 |