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Series GSE185504 Query DataSets for GSE185504
Status Public on Oct 09, 2021
Title miRNA profiling of colitis-associated dysplasia in animals submitted to the AOM/DSS model
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary The impact of the antiinflammatory agent 5-aminosalicylic acid (5-ASA) on the risk for colitis-associated colorectal cancer remains controversial. The chemopreventive activity of 5-ASA was evaluated in the Swiss Webster model of azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colitis-associated neoplasia. Mice were injected with AOM (7.4 mg/kg i.p.) and randomized to receive either vehicle or 5-ASA (75mg/kg) for the remainder of the study. Only untreated animals were used in this array.
DSS treatment began at 9 weeks of age and continued for 3 cycles. At the time of sacrifice (18 weeks of age), the entire colon and rectum were processed for histopathologic examination and microarray profiling. For information regarding the histopathological analysis, refer to Clapper ML, Gary MA, Coudry RA, Litwin S, Chang WC, Devarajan K, Lubet RA, Cooper HS. 5-aminosalicylic acid inhibits colitis-associated colorectal dysplasias in the mouse model of azoxymethane/dextran sulfate sodium-induced colitis. Inflamm Bowel Dis. 2008 Oct;14(10):1341-7. doi: 10.1002/ibd.20489. PMID: 18452197.
 
Overall design Mice were injected with AOM (7.4 mg/kg i.p.) and randomized to receive either vehicle or 5-ASA (75 mg/kg) for the remainder of the study. DSS treatment began at 9 weeks of age and continued for 3 cycles. At the time of sacrifice (18 weeks of age), the entire colon and rectum were processed for histopathologic examination.
 
Contributor(s) Fragoso MF, Fernandez GJ, Vanderveer L, Cooper H, Slifker M, Clapper M
Citation https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232113024
Submission date Oct 07, 2021
Last update date Oct 28, 2022
Contact name Mariana Franco Fragoso
E-mail(s) marifragoso@gmail.com, mariana.fragoso@fccc.edu
Organization name Fox Chase Cancer Center
Department Cancer Prevention and Control
Street address 333 Cottman Avenue
City Philadelphia
State/province PA
ZIP/Postal code 19111
Country USA
 
Platforms (1)
GPL30827 nCounter Mouse miRNA Assay
Samples (11)
GSM5616916 Inflamed-1
GSM5616917 Inflamed-2
GSM5616918 Inflamed-3
Relations
BioProject PRJNA769306

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185504_RAW.tar 70.0 Kb (http)(custom) TAR (of RCC)
Processed data included within Sample table

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