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Series GSE18558 Query DataSets for GSE18558
Status Public on Oct 13, 2010
Title Downregulation of Myc is essential for terminal erythroid maturation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Terminal differentiation of mammalian erythroid progenitors involves 4-5 cell divisions and induction of many erythroid important genes, followed by chromatin and nuclear condensation and enucleation. The protein levels of c-myc (Myc) are reduced dramatically during late stage erythroid maturation, coinciding with cell cycle arrest in G1-phase and enucleation, suggesting possible roles for c-myc in either or both of these processes. Here we demonstrate that ectopic Myc expression affects terminal erythroid maturation in a dose-dependent manner. Expression of Myc at physiological levels did not affect erythroid differentiation or cell cycle shutdown, but specifically blocked erythroid nuclear condensation and enucleation. Myc prevented deacetylation of several lysine residues in histones H3 and H4 that are normally deacetylated during erythroid maturation. When over-expressed at levels higher than the physiological range, Myc blocked erythroid differentiation and the cells continued to proliferate in cytokine-free, serum-containing culture medium with an early erythroblast morphology. These studies reveal an important dose-dependent function of Myc in regulating terminal maturation in mammalian erythroid cells. Our findings further support the emerging notion that Myc regulates chromatin structure by regulating global histone acetylation states.
 
Overall design Five groups with three biological replicates in each.
 
Contributor(s) Jayapal SR, Lee KL, Ji P, Kaldis P, Lodish HF, Lim B
Citation(s) 20940306
Submission date Oct 14, 2009
Last update date Jun 14, 2018
Contact name Senthil Raja Jayapal
E-mail(s) senthilraja@gis.a-star.edu.sg
Phone +65 64788187
Fax +65 6478-9005
Organization name Genome Institute of singapore
Department Stem cell and developmental biology
Lab Stem cell group 4
Street address #08-01, Genome, 60 Biopolis Street
City Singapore
ZIP/Postal code 138672
Country Singapore
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (15)
GSM541245 Wild type erythroblasts-uninfected 0hours 1
GSM541246 Wild type erythroblasts-uninfected 0hours 2
GSM541247 Wild type erythroblasts-uninfected 0hours 3
Relations
BioProject PRJNA121375

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18558_RAW.tar 3.1 Mb (http)(custom) TAR
GSE18558_non-normalized.txt.gz 2.6 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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