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Series GSE185859 Query DataSets for GSE185859
Status Public on Oct 15, 2021
Title Screening for potential target genes of hsa-mir-199a-1 and hsa-mir-4423 using Clariom D arrays
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We screened for target genes of hsa-mir-199a-1 and hsa-mir-4423 by transcriptome profiling. The miRNAs were overexpressed in HeLa cells by transient transfection of precursor miRNA expression plasmids. High-density Clariom D arrays for human were used for the gene expression analysis. This GEO entry provides the microarray data preprocessed according to the gene level workflow.
Overall design HeLa cells were transiently transfected with non-viral precursor miRNA expression plasmids from GeneCopoeia (HmiR0296-MR04 and HmiR1083-MR04, respectively). As negative control, a plasmid with scrambled sequence (CmiR0001-MR04, GeneCopoeia) was used. Total RNA was isolated 24 h (n=2 replicates) and 48 h (n=4 replicates) following transfection. For all 18 RNA samples, transcript levels were measured with Clariom D arrays.
Contributor(s) Hecker M, Koczan D, Boxberger N, Zettl UK
Citation(s) 35561450
Submission date Oct 13, 2021
Last update date May 20, 2022
Contact name Michael Hecker
Organization name University of Rostock
Department Department of Neurology
Lab Division of Neuroimmunology
Street address Gehlsheimer Str. 20
City Rostock
ZIP/Postal code 18147
Country Germany
Platforms (1)
GPL23126 [Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version]
Samples (18)
GSM5624842 negative control, 24 h post-transfection, replicate 1
GSM5624843 negative control, 24 h post-transfection, replicate 2
GSM5624844 negative control, 48 h post-transfection, replicate 1
BioProject PRJNA771013

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Supplementary file Size Download File type/resource
GSE185859_RAW.tar 367.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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