 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 15, 2021 |
| Title |
Screening for potential target genes of hsa-mir-199a-1 and hsa-mir-4423 using Clariom D arrays |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We screened for target genes of hsa-mir-199a-1 and hsa-mir-4423 by transcriptome profiling. The miRNAs were overexpressed in HeLa cells by transient transfection of precursor miRNA expression plasmids. High-density Clariom D arrays for human were used for the gene expression analysis. This GEO entry provides the microarray data preprocessed according to the gene level workflow.
|
| |
|
| Overall design |
HeLa cells were transiently transfected with non-viral precursor miRNA expression plasmids from GeneCopoeia (HmiR0296-MR04 and HmiR1083-MR04, respectively). As negative control, a plasmid with scrambled sequence (CmiR0001-MR04, GeneCopoeia) was used. Total RNA was isolated 24 h (n=2 replicates) and 48 h (n=4 replicates) following transfection. For all 18 RNA samples, transcript levels were measured with Clariom D arrays.
|
| |
|
| Contributor(s) |
Hecker M, Koczan D, Boxberger N, Zettl UK |
| Citation(s) |
35561450 |
| Submission date |
Oct 13, 2021 |
| Last update date |
May 20, 2022 |
| Contact name |
Michael Hecker |
| E-mail(s) |
michael.hecker@rocketmail.com
|
| Organization name |
University of Rostock
|
| Department |
Department of Neurology
|
| Lab |
Division of Neuroimmunology
|
| Street address |
Gehlsheimer Str. 20
|
| City |
Rostock |
| ZIP/Postal code |
18147 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
|
| Samples (18)
|
| GSM5624842 |
negative control, 24 h post-transfection, replicate 1 |
| GSM5624843 |
negative control, 24 h post-transfection, replicate 2 |
| GSM5624844 |
negative control, 48 h post-transfection, replicate 1 |
| GSM5624845 |
negative control, 48 h post-transfection, replicate 2 |
| GSM5624846 |
negative control, 48 h post-transfection, replicate 3 |
| GSM5624847 |
negative control, 48 h post-transfection, replicate 4 |
| GSM5624848 |
mir-199a-1 overexpression, 24 h post-transfection, replicate 1 |
| GSM5624849 |
mir-199a-1 overexpression, 24 h post-transfection, replicate 2 |
| GSM5624850 |
mir-199a-1 overexpression, 48 h post-transfection, replicate 1 |
| GSM5624851 |
mir-199a-1 overexpression, 48 h post-transfection, replicate 2 |
| GSM5624852 |
mir-199a-1 overexpression, 48 h post-transfection, replicate 3 |
| GSM5624853 |
mir-199a-1 overexpression, 48 h post-transfection, replicate 4 |
| GSM5624854 |
mir-4423 overexpression, 24 h post-transfection, replicate 1 |
| GSM5624855 |
mir-4423 overexpression, 24 h post-transfection, replicate 2 |
| GSM5624856 |
mir-4423 overexpression, 48 h post-transfection, replicate 1 |
| GSM5624857 |
mir-4423 overexpression, 48 h post-transfection, replicate 2 |
| GSM5624858 |
mir-4423 overexpression, 48 h post-transfection, replicate 3 |
| GSM5624859 |
mir-4423 overexpression, 48 h post-transfection, replicate 4 |
|
| Relations |
| BioProject |
PRJNA771013 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE185859_RAW.tar |
367.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...