|
| Status |
Public on Oct 15, 2021 |
| Title |
Engineering Extracellular Vesicles by Three-dimensional Dynamic Culture of Human Mesenchymal Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In current study, hMSCs were grown as 3D aggregates under wave motion to promote EV secretion (3D EVs). mRNA sequencing reveals global transcriptome alterations (e.g., upregulated Wnt, TNF, and Hippo signaling and downregulated cellular senescence) for 3D aggregates.
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| |
|
| Overall design |
MSC cell were cultured under either 2D, 3D or hypoxia conditions, and harvested for mRNA-seq.
|
| |
|
| Contributor(s) |
Sun L, Yuan X |
| Citation(s) |
35716062 |
| Submission date |
Oct 13, 2021 |
| Last update date |
Apr 09, 2023 |
| Contact name |
li sun |
| E-mail(s) |
lsun2@fsu.edu, davidgmeckes@gmail.com
|
| Organization name |
Florida State University
|
| Department |
College of medicine
|
| Street address |
1115 west call st.
|
| City |
Tallahassee |
| State/province |
FL |
| ZIP/Postal code |
32306 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (18)
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|
| Relations |
| BioProject |
PRJNA771080 |
| SRA |
SRP341267 |
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