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| Status |
Public on Oct 20, 2021 |
| Title |
Lysyl hydroxylase 2 deficiency promotes filopodia formation and fibroblast migration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Lysyl hydroxylase 2 (LH2) regulates the intermolecular cross-linking of collagen molecules. Accumulation of LH2-modified collagen, which is highly stable and resistant to collagenase cleavage, is one of the causes of fibrosis. In this study, we established LH2-deficient fibroblasts and first analyzed morphology of LH2-deficient fibroblasts by microscopy. LH2-deficient fibroblasts showed a dramatic increase in filopodial dynamics and cell migration, which were supported by time-lapse video microscopy and Immunocytochemistry, and gene expression profiling data. Understanding the precise role of phenotype in LH2-deficient cells may be helpful to define the pathogenesis of fibrosis.
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| Overall design |
Data shows the comparison of differentially expressed genes in LH2 cKO TTFs in culture medium with tamoxifen (LH2 cKO_no.1) vs. LH2 cKO TTFs in culture medium without tamoxifen (LH2 cKO_no.2) after 24h tamoxifen treatent.
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| Contributor(s) |
Nozaki R, Kasamatsu A, Moss J, Uzawa K |
| Citation(s) |
34875533 |
| Submission date |
Oct 19, 2021 |
| Last update date |
Jan 20, 2022 |
| Contact name |
Ryunosuke Nozaki |
| E-mail(s) |
nozaki.r@chiba-u.jp
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| Organization name |
Graduate School of Medecine, Chiba university
|
| Department |
Depertment of Oral science
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| Street address |
1-8-1 Inohana, Chuo-ku, Chiba, Japan
|
| City |
Chiba City |
| State/province |
Chiba Prefecture |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA772615 |
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