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Series GSE18795 Query DataSets for GSE18795
Status Public on Oct 30, 2009
Title Expression data from zebrafish embryos homozygous mutant for the cohesin subunit Rad21
Organism Danio rerio
Experiment type Expression profiling by array
Summary Rad21 is a subunit of cohesin. The main function of cohesin is to hold replicated chromosomes together until cells divide, but it also plays a role in gene expression. To find out which genes might be regulated by cohesin, a study was conducted to look for global changes in gene expression in zebrafish embryos lacking cohesin component Rad21.
The zebrafish Rad21 mutant used for expression analysis was rad21nz171, an allele isolated in a forward genetic screen for regulators of runx1.
 
Overall design RNA from rad21nz171 mutant and wild type zebrafish embryos collected at 24 hours post-fertilization (h.p.f.) and 48 h.p.f. was hybridized to Affymetrix microarrays (Gene Chip zebrafish genome arrays cat. no. 900488). Four pools of 50 embryos for each genotype and time point were used as the RNA source, and RNA from each pool was hybridized independently such that the experiment had four biological replicates.
 
Contributor(s) Horsfield JA, Print CG, Wiilliams L, Crosier PS
Citation(s) 20553708
Submission date Oct 29, 2009
Last update date Jan 25, 2018
Contact name Julia Horsfield
E-mail(s) julia.horsfield@otago.ac.nz
Organization name University of Otago
Department Pathology Department
Lab Chromosome Structure and Development Group
Street address Cnr Hanover and Great King Streets
City Dunedin
ZIP/Postal code 9016
Country New Zealand
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (16)
GSM466295 Embryo_Rad21 mutant_24hpf_biological rep 1
GSM466296 Embryo_Rad21 mutant_24hpf_biological rep 2
GSM466297 Embryo_Rad21 mutant_24hpf_biological rep 3
Relations
BioProject PRJNA121201

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Supplementary file Size Download File type/resource
GSE18795_RAW.tar 33.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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