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| Status |
Public on Jan 20, 2022 |
| Title |
Genome-wide identification of transcriptional targets for H4K12la in total brain cells and microglia of WT and 5XFAD mice |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In this study, we found that H4K12la level is elevated in plaque-adjacent microglia of 5XFAD mice. To further explore the potential functional significance of H4K12la in AD, we performed genome-wide cleavage under targets and tagmentation (CUT&Tag) analysis to identify candidate genes regulated by H4K12la in total brain cells and microglia. Following CUT&Tag, H4K12la-associated DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE.
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| Overall design |
Examination of transcriptional targets for H4K12la in total brain cells and microglia of WT and 5XFAD mice.
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| Contributor(s) |
Pan R, He L, Liu X |
| Citation missing |
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| Submission date |
Nov 14, 2021 |
| Last update date |
Mar 17, 2022 |
| Contact name |
Lin He |
| E-mail(s) |
linhe@bjmu.edu.cn
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| Organization name |
Peking University
|
| Street address |
Xueyuan Road 38
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| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA780259 |
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