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| Status |
Public on Nov 25, 2021 |
| Title |
Interferon (IFN)-γ promotes monocyte-mediated lung injury during influenza pathogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Influenza A Virus (IAV) triggers an exuberant host response that promotes acute lung injury. However, the determinants of the pathological host response to IAV remain incompletely understood. In the current study, we identified interferon (IFN)-γ-regulated subset of monocytes, CCR2+ monocytes, as a driver of lung damage during IAV pathogenesis. IFN-γ regulated the recruitment and inflammatory phenotype of CCR2+ monocytes, and CCR2 (CCR2-/-) and IFN-γ (IFN-γ-/-) deficient mice exhibited reduced lung inflammation, pathology, and increased resistance against bacterial co-infection by Streptococcus pneumoniae (Spn). Adoptive transfer of WT (IFN-γR1+), but not IFN-γR1 deficient (IFN-γR1-) CCR2+ monocytes, restored the wild-type (WT)-like pathological phenotype of lung damage in IAV-infected CCR2-/- mice. The CD8+ T cells were the most significant source of IFN-γ in IAV-infected lungs. Collectively, our data highlight that IFN-γ regulates CCR2+ monocyte-mediated lung pathology during IAV pathogenesis.
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| Overall design |
scRNAseq profiles of mock, 7 days post-viral infection
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| Contributor(s) |
Khan MN, Guo K |
| Citation(s) |
35235782 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI143741 |
Pathogenic role of IL-17 response in Streptococcus pneumoniae nasopharyngeal pathogenesis during an influenza virus co-infection |
UNIVERSITY OF NORTH DAKOTA |
Mohammad Nadeem Khan |
| R21 AI151522 |
Utilization of the adjuvant effect of CRM197 protein to develop a trivalentprotein-vaccine against Streptococcus pneumoniae infections |
UNIVERSITY OF NORTH DAKOTA |
Mohammad Nadeem Khan |
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| Submission date |
Nov 23, 2021 |
| Last update date |
Mar 03, 2022 |
| Contact name |
Kai Guo |
| E-mail(s) |
guokai8@gmail.com
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| Organization name |
University of North Dakota
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| Street address |
1301 N Columbia Rd Stop 9037
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| City |
Grand Forks |
| State/province |
ND |
| ZIP/Postal code |
58202 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA782962 |
| SRA |
SRP347430 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE189407_RAW.tar |
29.4 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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