|
| Status |
Public on Jul 01, 2012 |
| Title |
Gene expression profiling of Plasmodium falciparum after co-culture with NK cells |
| Platform organisms |
Plasmodium falciparum; Anopheles gambiae |
| Sample organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim of the study was to determine the effect of natural killer (NK) cells on the global gene expression in Plasmodium falciparum.
|
| |
|
| Overall design |
FCR3-CSA-infected red blood cells (RBCs) were co-cultured for 24h with NK92. Prior to RNA extraction, the parasitized RBCs were separated via Ficoll from the lymphocyte fraction. Control cultures of parasites without NK cells were cultivated in parallel. In addition, the effect of the separation method was determined via a Ficoll control culture of FCR3-CSA that was passed over a Ficoll gradient. All three treatments were performed in triplicate. The RNA was reverse-transcribed and hybridized onto microarrays.
|
| |
|
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 13, 2009 |
| Last update date |
Jan 24, 2013 |
| Contact name |
Evelyn Boettger |
| E-mail(s) |
evelyn-boettger@hotmail.de
|
| Organization name |
Institute of Tropical Medicine
|
| Street address |
Wilhelmstrasse 27
|
| City |
Tuebingen |
| ZIP/Postal code |
72074 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL1321 |
[Plasmodium_Anopheles] Affymetrix Plasmodium/Anopheles Genome Array |
|
| Samples (9)
|
| GSM470353 |
Plasmodium falciparum, parasite control, A |
| GSM470357 |
Plasmodium falciparum, parasite control, B |
| GSM470384 |
Plasmodium falciparum, parasite control, C |
| GSM470386 |
Plasmodium falciparum, co-cultured with NK cells, A |
| GSM470388 |
Plasmodium falciparum, co-cultured with NK cells, B |
| GSM470389 |
Plasmodium falciparum, co-cultured with NK cells, C |
| GSM470391 |
Plasmodium falciparum, Ficoll control, A |
| GSM470392 |
Plasmodium falciparum, Ficoll control, B |
| GSM470405 |
Plasmodium falciparum, Ficoll control, C |
|
| Relations |
| BioProject |
PRJNA121073 |
Less...
More...